Effects of acidosis on Ca2+ sensitivity of contractile elements in intact ferret myocardium.

Abstract

We investigated the effects of acidosis on the intracellular Ca2+ concentration ([Ca2+]i) and contractile properties of intact mammalian cardiac muscle during tetanic and twitch contractions. Aequorin was injected into ferret papillary muscles, and the [Ca2+]i and tension were simultaneously measured. Acidosis was attained by increasing the CO2 concentration in the bicarbonate (20 mM)-buffered Tyrode solution from 5% (pH 7.35, control) to 15% (pH 6.89, acidosis). Tetanic contraction was produced by repetitive stimulation of the preparation following treatment with 5 microM ryanodine. The relationship between [Ca2+]i and tension was measured 6 s after the onset of the stimulation and was fitted using the Hill equation. Acidosis decreased the maximal tension to 81 +/- 2% of the control and shifted the [Ca2+]i-tension relationship to the right by 0.18 +/- 0.01 pCa units. During twitch contraction, a quick shortening of muscle length from the length at which developed tension became maximal (Lmax) to 92% Lmax produced a transient change in the [Ca2+]i (extra Ca2+). The magnitude of the extra Ca2+ was dependent on the [Ca2+]i immediately before the length change, suggesting that the extra Ca2+ is related to the amount of troponin-Ca complex. Acidosis decreased the normalized extra Ca2+ to [Ca2+]i immediately before the length change, which indicates that the amount of Ca2+ bound to troponin C is less when [Ca2+]i is the same as in the control. The decrease in the Ca2+ binding to troponin C explains the decrease in tetanic and twitch contraction, and mechanical stress applied to the preparation induced less [Ca2+]i change in acidosis.

• Authors: K Komukai, T Ishikawa, S Kurihara
• Journal: The American journal of physiology (Am J Physiol) Vol. 274 Issue 1 Pg. H147-54 (01 1998) ISSN: 0002-9513 [Print] United States
• PMID: 9458863 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ryanodine
• Aequorin
• Calcium

Topics

• Acidosis
• Aequorin
• Animals
• Calcium (pharmacology, physiology)
• Electric Stimulation
• Ferrets
• In Vitro Techniques
• Myocardial Contraction (drug effects, physiology)
• Papillary Muscles (drug effects, physiology, physiopathology)
• Ryanodine (pharmacology)
• Time Factors