Identification and characterization of a protein kinase-interacting protein encoded by the Autographa californica nuclear polyhedrosis virus.

Abstract

We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PKIP is a 19.2-kDa protein and appears to stimulate the activity of the viral protein kinase-1 in vitro. Northern blotting analysis revealed that pkip is a late gene. Attempts to plaque purify a pkip- virus were unsuccessful.

• Authors: X Fan, J R McLachlin, R F Weaver
• Journal: Virology (Virology) Vol. 240 Issue 2 Pg. 175-82 (Jan 20 1998) ISSN: 0042-6822 [Print] United States
• PMID: 9454690 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Carrier Proteins
• Viral Proteins
• pkip protein, Autographa californica nucleopolyhedrovirus

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Blotting, Northern
• Carrier Proteins (chemistry, isolation & purification)
• Enzyme Activation
• Genetic Vectors
• Molecular Sequence Data
• Nucleopolyhedroviruses (genetics)
• Sequence Alignment
• Spodoptera (virology)
• Viral Proteins (metabolism)
• Yeasts (genetics)