Abstract | BACKGROUND/AIMS: Hepatic stellate cells represent the principal matrix-synthesising cells of damaged liver and are targets of a number of cytokines currently under investigation. The study analyses the effects of tumour necrosis factor-alpha and interferon-gamma on proliferation, "activation" and protein synthesis of hepatic stellate cells. METHODS: Primary cultures of hepatic stellate cells were exposed to tumour necrosis factor-alpha and interferon-gamma. Cell proliferation was studied by 3H-thymidine and bromo-deoxy- uridine incorporation. Protein synthesis was analysed using immunoprecipitation, Western- and Northern blotting techniques. RESULTS: CONCLUSIONS: Tumour necrosis factor-alpha and interferon-gamma decrease proliferation of hepatic stellate cells, while "activation" of hepatic stellate cells and synthesis of proteins involved in matrix metabolism are regulated in a differential, cytokine-specific manner, suggesting that both cytokines play an important role in liver repair.
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Authors | T Knittel, L Müller, B Saile, G Ramadori |
Journal | Journal of hepatology
(J Hepatol)
Vol. 27
Issue 6
Pg. 1067-80
(Dec 1997)
ISSN: 0168-8278 [Print] Netherlands |
PMID | 9453433
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Extracellular Matrix Proteins
- Protease Inhibitors
- Tumor Necrosis Factor-alpha
- Interferon-gamma
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Topics |
- Adipocytes
(drug effects, metabolism)
- Animals
- Cell Division
(drug effects)
- Extracellular Matrix Proteins
(biosynthesis)
- Interferon-gamma
(pharmacology)
- Liver
(cytology, drug effects)
- Protease Inhibitors
(metabolism)
- Rabbits
- Rats
- Rats, Wistar
- Tumor Necrosis Factor-alpha
(pharmacology)
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