The t(2;5)-associated p80 NPM/ALK fusion protein in nodal and cutaneous CD30+ lymphoproliferative disorders.

Abstract

A high percentage of extracutaneous CD30+ anaplastic large cell lymphomas (nodal ALCL) carry a specific chromosomal translocation, t(2;5) (p23;q35), that results in abnormal expression of p80 NPM/ALK chimeric protein (p80). The protein p80 may be detected by immunohistochemistry using polyclonal (anti-p80) or monoclonal (ALK1) antibody directed against the ALK epitope. Although nodal ALCL, primary cutaneous ALCL, and lymphomatoid papulosis type A (lyp A) have similar histologic and immunohistochemical features, the expression of p80 in these cutaneous lesions has not been extensively studied. We immunostained tissues from 10 nodal ALCL, 8 primary cutaneous ALCL, 24 lyp A, and positive and negative controls using polyclonal rabbit anti-p80 and the avidin-biotin-peroxidase labeling method. Reactivity was determined by comparing staining intensity to positive controls [4 nodal ALCL with t(2;5)] and negative controls (21 non-ALCL lymphomas). Only cutaneous lesions staining positively with anti-p80 were further studied with the monoclonal antibody ALK1 and reverse transcription polymerase chain reaction (RT-PCR) for p80 messenger RNA. All positive controls (4/4), but none of the negative controls (0/21) nor lyp A (0/24), were immunoreactive for anti-p80. Sixty percent (6/10) of nodal ALCL and a single case (12%) of primary cutaneous ALCL were immunoreactive for anti-p80. In this exceptional cutaneous lesion, although we did not find NPM/ALK by RT-PCR, we detected strong expression of ALK using ALK1. We conclude that t(2;5) is rarely involved in the pathogenesis of cutaneous CD30+ lymphoproliferative disorders.

• Authors: L D Su, B Schnitzer, C W Ross, M Vasef, S Mori, M Shiota, D Y Mason, K Pulford, J T Headington, T P Singleton
• Journal: Journal of cutaneous pathology (J Cutan Pathol) Vol. 24 Issue 10 Pg. 597-603 (Nov 1997) ISSN: 0303-6987 [Print] United States
• PMID: 9449486 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ki-1 Antigen
• ALK protein, human
• Anaplastic Lymphoma Kinase
• Protein-Tyrosine Kinases
• Receptor Protein-Tyrosine Kinases

Topics

• Anaplastic Lymphoma Kinase
• Chromosomes, Human, Pair 2
• Chromosomes, Human, Pair 5
• Humans
• Immunohistochemistry
• Ki-1 Antigen (analysis)
• Lymphoproliferative Disorders (genetics, metabolism, pathology)
• Polymerase Chain Reaction
• Protein-Tyrosine Kinases (genetics, metabolism)
• Receptor Protein-Tyrosine Kinases
• Transcription, Genetic
• Translocation, Genetic