HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Vaccination with protein-conjugated and native type 3 capsular polysaccharide in an ethanol-fed rat model of pneumococcal pneumonia.

Abstract
A chronic ethanol-fed rat model was used to determine the effect of alcohol ingestion on production of antibody to type 3 pneumococcal capsular polysaccharide. Sprague-Dawley rats were fed a liquid diet containing 36% of calories as ethanol (ethanol-fed), an isocaloric diet containing dextrin-maltose (pair-fed) or standard rat chow (chow-fed). After 7 days of feeding, the rats were vaccinated subcutaneously with placebo or with either 25 microg of type 3 pneumococcal capsular polysaccharide (SpnCP) or 5 microg of SpnCP linked to the protein carrier CRM197 (SpnCP/CRM197). Rats given the conjugated vaccine received a booster injection 14 days later. Maximum antibody titers were observed six days postvaccination for rats given SpnCP alone and 21 days postvaccination for rats given SpnCP/CRM197. All rats were infected transtracheally with 2-3 times the expected lethal dose50 for each feeding group of type 3 Streptococcus pneumoniae on the day of peak antibody titers. Mortality was recorded for a 10-day period. Vaccination with SpnCP increased survival of ethanol- and chow-fed, but not pair-fed rats. This protection was only statistically significant in the chow-fed group (p < 0.01). Vaccination with SpnCP/CRM197 moderately increased survival of rats in all three feeding groups, but this was not statistically significant in any of them.
AuthorsJ L Henriksen, L C Preheim, M J Gentry
JournalAlcoholism, clinical and experimental research (Alcohol Clin Exp Res) Vol. 21 Issue 9 Pg. 1630-7 (Dec 1997) ISSN: 0145-6008 [Print] England
PMID9438523 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Polysaccharides, Bacterial
  • Vaccines, Conjugate
  • Ethanol
Topics
  • Alcohol Drinking (immunology)
  • Animals
  • Antibody Formation
  • Disease Models, Animal
  • Disease Susceptibility (immunology)
  • Ethanol (administration & dosage)
  • Feeding Behavior
  • Pneumonia, Pneumococcal (immunology)
  • Polysaccharides, Bacterial (immunology)
  • Rats
  • Rats, Sprague-Dawley
  • Streptococcus pneumoniae (immunology)
  • Vaccination (methods)
  • Vaccines, Conjugate (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: