Abstract |
A chronic ethanol-fed rat model was used to determine the effect of alcohol ingestion on production of antibody to type 3 pneumococcal capsular polysaccharide. Sprague-Dawley rats were fed a liquid diet containing 36% of calories as ethanol ( ethanol-fed), an isocaloric diet containing dextrin-maltose (pair-fed) or standard rat chow (chow-fed). After 7 days of feeding, the rats were vaccinated subcutaneously with placebo or with either 25 microg of type 3 pneumococcal capsular polysaccharide (SpnCP) or 5 microg of SpnCP linked to the protein carrier CRM197 (SpnCP/ CRM197). Rats given the conjugated vaccine received a booster injection 14 days later. Maximum antibody titers were observed six days postvaccination for rats given SpnCP alone and 21 days postvaccination for rats given SpnCP/ CRM197. All rats were infected transtracheally with 2-3 times the expected lethal dose50 for each feeding group of type 3 Streptococcus pneumoniae on the day of peak antibody titers. Mortality was recorded for a 10-day period. Vaccination with SpnCP increased survival of ethanol- and chow-fed, but not pair-fed rats. This protection was only statistically significant in the chow-fed group (p < 0.01). Vaccination with SpnCP/ CRM197 moderately increased survival of rats in all three feeding groups, but this was not statistically significant in any of them.
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Authors | J L Henriksen, L C Preheim, M J Gentry |
Journal | Alcoholism, clinical and experimental research
(Alcohol Clin Exp Res)
Vol. 21
Issue 9
Pg. 1630-7
(Dec 1997)
ISSN: 0145-6008 [Print] England |
PMID | 9438523
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Polysaccharides, Bacterial
- Vaccines, Conjugate
- Ethanol
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Topics |
- Alcohol Drinking
(immunology)
- Animals
- Antibody Formation
- Disease Models, Animal
- Disease Susceptibility
(immunology)
- Ethanol
(administration & dosage)
- Feeding Behavior
- Pneumonia, Pneumococcal
(immunology)
- Polysaccharides, Bacterial
(immunology)
- Rats
- Rats, Sprague-Dawley
- Streptococcus pneumoniae
(immunology)
- Vaccination
(methods)
- Vaccines, Conjugate
(immunology)
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