Abstract |
A one-step purification procedure will yield monoclonal antibodies from cell-culture supernatants and ascites fluids. The chromatographic adsorbent is thiophilic argose, i.e., beaded agarose gel coupled with ligands of thiophilic nature, often with a sulfone group and a sulfur atom. The chromatographic procedure is simply adsorption, wash, elution. The procedure is simple, efficient, and inexpensive.
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Authors | T C Bøg-Hansen |
Journal | Molecular biotechnology
(Mol Biotechnol)
Vol. 8
Issue 3
Pg. 279-81
(Dec 1997)
ISSN: 1073-6085 [Print] United States |
PMID | 9438262
(Publication Type: Journal Article)
|
Chemical References |
- Antibodies, Monoclonal
- Culture Media
- Sepharose
|
Topics |
- Antibodies, Monoclonal
(isolation & purification)
- Ascitic Fluid
- Cells, Cultured
- Culture Media
- Sepharose
|