HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Oxidized heme proteins in an animal model of hemochromatosis.

Abstract
Hemichrome formation resulting from oxidation of heme proteins has been proposed as sensitive indicator of iron-induced oxidative stress. The Heme Protein Spectra Analysis Program was used to quantitate oxidized heme proteins (OHP) in chronic hepatic iron overload in vivo. Rats were fed a diet containing carbonyl iron for 14 months with or without vitamin E supplementation. A 30-fold increase in hepatic iron concentration was observed in rats fed the iron-containing diet. At baseline, total OHP and TBARS were significantly elevated in iron-loaded livers but no increase in hemichrome was seen. During 3 h of spontaneous oxidation, hemichrome formation increased significantly in iron-loaded livers compared to controls. Although supplemental vitamin E was associated with lower levels of OHP and TBARS in iron-loaded livers at baseline, it did not significantly inhibit hemichrome formation during in vitro oxidation. In conclusion, an increase in hemichrome formation in iron-loaded livers was observed only during oxidation in vitro. These results suggest that total OHP is more sensitive than hemichrome formation as an indicator of oxidative stress in this in vivo model of iron overload.
AuthorsK E Brown, C A Knudsen
JournalFree radical biology & medicine (Free Radic Biol Med) Vol. 24 Issue 2 Pg. 239-44 (Jan 15 1998) ISSN: 0891-5849 [Print] United States
PMID9433898 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Hemeproteins
  • Thiobarbituric Acid Reactive Substances
  • hemichrome
  • Vitamin E
  • Iron
Topics
  • Animals
  • Diet
  • Disease Models, Animal
  • Hemeproteins (metabolism)
  • Hemochromatosis (chemically induced, metabolism)
  • Iron (administration & dosage, metabolism)
  • Kinetics
  • Liver (metabolism)
  • Male
  • Oxidation-Reduction
  • Rats
  • Rats, Sprague-Dawley
  • Thiobarbituric Acid Reactive Substances (metabolism)
  • Vitamin E (administration & dosage, blood, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: