HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Transcription of the Rhodobacter sphaeroides cycA P1 promoter by alternate RNA polymerase holoenzymes.

Abstract
These experiments sought to identify what form of RNA polymerase transcribes the P1 promoter for the Rhodobacter sphaeroides cytochrome c2 gene (cycA). In vitro, cycA P1 was recognized by an RNA polymerase holoenzyme fraction that transcribes several well-characterized Escherichia coli heat shock (sigma32) promoters. The in vivo effects of mutations flanking the transcription initiation site (+1) also suggested that cycA P1 was recognized by an RNA polymerase similar to E. coli Esigma32. Function of cycA P1 was not altered by mutations more than 35 bp upstream of position +1 or by alterations downstream of -7. A point mutation at position -34 that is towards the E. coli Esigma32 -35 consensus sequence (G34T) increased cycA P1 activity approximately 20-fold, while several mutations that reduced or abolished promoter function changed highly conserved bases in presumed -10 or -35 elements. In addition, cycA P1 function was retained in mutant promoters with a spacer region as short as 14 nucleotides. When either wild-type or G34T promoters were incubated with reconstituted RNA polymerase holoenzymes, cycA P1 transcription was observed only with samples containing either a 37-kDa subunit that is a member of the heat shock sigma factor family (Esigma37) or a 38-kDa subunit that also allows core RNA polymerase to recognize E. coli heat shock promoters (Esigma38). (R. K. Karls, J. Brooks, P. Rossmeissl, J. Luedke, and T. J. Donohue, J. Bacteriol. 180:10-19, 1998).
AuthorsB J MacGregor, R K Karls, T J Donohue
JournalJournal of bacteriology (J Bacteriol) Vol. 180 Issue 1 Pg. 1-9 (Jan 1998) ISSN: 0021-9193 [Print] United States
PMID9422585 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Coenzymes
  • Cytochrome c Group
  • Heat-Shock Proteins
  • Recombinant Fusion Proteins
  • Sigma Factor
  • Transcription Factors
  • heat-shock sigma factor 32
  • Cytochromes c2
  • cytochrome c(3)
  • RNA polymerase Esigma(38)
  • DNA-Directed RNA Polymerases
Topics
  • Base Sequence
  • Coenzymes (metabolism)
  • Cytochrome c Group (genetics)
  • Cytochromes c2
  • DNA-Directed RNA Polymerases (metabolism)
  • Heat-Shock Proteins (metabolism)
  • Molecular Sequence Data
  • Point Mutation
  • Promoter Regions, Genetic (genetics)
  • Recombinant Fusion Proteins
  • Rhodobacter sphaeroides (genetics)
  • Sequence Deletion
  • Sigma Factor (metabolism)
  • Transcription Factors
  • Transcription, Genetic (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: