Substance P endopeptidase activity in the rat spinal cord following injury: influence of the new anti-oxidant compound H 290/51.

Abstract

The influence of the new antioxidant compound H-290/51 was examined on the substance P endopeptidase (SPE) activity in a rat model of spinal cord injury. This compound (H-290/51) has neuro-protective effects on edema and cell changes in this model. Infliction of trauma to the cord by making an incision into the right dorsal horn of the T10-11 segment resulted in a marked upregulation of SPE in the segments rostral to the lesion. On the other hand, the injured and adjacent caudal segments exhibited a marked down-regulation of the enzyme activity. Pretreatment with H 290/51 increased the SPE activity in the T9 segment but downregulated the enzyme activity in the T10-11 and T12 segments. The drug induced enzyme activity change was not further influenced by the trauma of the cord. The results indicate that a focal trauma induces widespread alterations in spinal cord SPE activity which can be influenced by the anti-oxidant drug H 290/51, suggesting that SPE is somehow involved in cell injury.

• Authors: M Thörnwall, H S Sharma, T Gordh, P O Sjöquist, F Nyberg
• Journal: Acta neurochirurgica. Supplement (Acta Neurochir Suppl) Vol. 70 Pg. 212-5 ( 1997) ISSN: 0065-1419 [Print] Austria
• PMID: 9416325 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• H290-51
• Indoles
• Neuroprotective Agents
• Metalloendopeptidases
• substance P degrading enzyme

Topics

• Animals
• Antioxidants (therapeutic use)
• Indoles (therapeutic use)
• Metalloendopeptidases (metabolism)
• Neuroprotective Agents (therapeutic use)
• Oxidative Stress (drug effects)
• Radioimmunoassay
• Rats
• Rats, Sprague-Dawley
• Reference Values
• Sensitivity and Specificity
• Spinal Cord (enzymology)
• Spinal Cord Injuries (enzymology)