Protein degradation in red cells exposed to 2,2'-azo-bis(2-amidinopropane) derived radicals.

Abstract

Extensive proteolysis is observed when red blood cells are exposed to free radicals produced in the thermolysis of 2,2'-azo-bis(2-amidinopropane). It is evaluated that nearly one amino terminal group is produced by each free radical introduced into the system. These groups are considered to arise mainly from band 3 fragmentation due to the action of red cell proteinases. Protein fragmentation takes place prior to significant hemolysis or lipid peroxidation, as evaluated by thiobarbituric acid-reactive substances measurements.

• Authors: G Celedón, V Lips, C Alvarado, M Cortés, E A Lissi, G González
• Journal: Biochemistry and molecular biology international (Biochem Mol Biol Int) Vol. 43 Issue 5 Pg. 1121-7 (Dec 1997) ISSN: 1039-9712 [Print] England
• PMID: 9415821 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amidines
• Blood Proteins
• Free Radicals
• Oxidants
• Peroxides
• perhydroxyl radical
• 2,2'-azobis(2-amidinopropane)
• Endopeptidases

Topics

• Amidines (pharmacology)
• Blood Proteins (drug effects, metabolism)
• Endopeptidases (blood)
• Erythrocyte Membrane (drug effects, enzymology)
• Erythrocytes (drug effects, enzymology, metabolism)
• Free Radicals (pharmacology)
• Hemolysis (drug effects)
• Humans
• Hydrolysis (drug effects)
• Lipid Peroxidation (drug effects)
• Oxidants (pharmacology)
• Peroxides (pharmacology)