Thrombin has a bimodal effect on glioma cell growth.

Abstract

Using rat glioma C6 cells as a model, we have found a bimodal effect of alpha-thrombin on cell growth. In C6 cells treated with alpha-thrombin at concentrations from 0.02 nM to 1.0 nM, inhibition of cell proliferation was noted. Because the thrombin receptor agonist peptide TRAP-6 also induced inhibition of cell proliferation and the thrombin receptor antagonist peptide T1 prevented the inhibitory effect of alpha-thrombin on C6 glioma cell growth, thrombin receptor involvement in antiproliferative action of alpha-thrombin in C6 glioma cells is highly likely. However, stimulation of cell proliferation observed when C6 cells were treated with alpha-thrombin at higher doses (> 1.0 nM) seems to be mediated by as yet undefined thrombin receptor-independent biochemical mechanisms.

• Authors: H Schafberg, G Nowak, R Kaufmann
• Journal: British journal of cancer (Br J Cancer) Vol. 76 Issue 12 Pg. 1592-5 ( 1997) ISSN: 0007-0920 [Print] England
• PMID: 9413947 (Publication Type: Journal Article)

Chemical References

• Peptide Fragments
• Receptors, Thrombin
• thrombin receptor peptide (42-47)
• DNA
• Thrombin

Topics

• Animals
• Cell Division (drug effects)
• DNA (biosynthesis)
• Glioma (pathology)
• Peptide Fragments (pharmacology)
• Rats
• Receptors, Thrombin (drug effects, physiology)
• Thrombin (pharmacology)
• Tumor Cells, Cultured