[Effect of phenylalanine and alpha-tocopherol on ubiquinone and ubichromenol metabolism in the liver of E-avitaminotic rats in vitro].

Abstract

When incubating the liver pulp of vitamin E-deficiency rats with 2-14C-sodium acetate and phenylalanine for an hour the content and radioactivity of ubiquinone and ubichromenol increase as compared to the control level. In this respect phenylalanine in in a dose of 60 and 120 mumol per 1 g of the liver pulp is most effective. Under conditions of these experiments incubation for 3 h causes no noticable changes in the content and metabolism of ubiquinone and ubichromenol in the liver pulp of vitamin E-deficiency rats as compared to the control level. Introduction of different amounts of alpha-tocopherol into the incubation medium results 3 h after an increase in concentration and radioactivity of ubiquinone, the alpha-tocopherol dose of 116 nmol being the most effective. An increase in the concentration and radioactivity of ubichromenol occurs at lower doses of exogenous alpha-tocopherol (58 nmol). All the used doses of alpha-tocopherol cause a decrease in intensity of radio-label incorporation into the unsaponifiable fraction of vitamin E-deficiency rat liver incubated for 3 h. Phenylalanine also results in a decrease of these values with the system incubation for an hour and produces no changes in them in the 3 h incubation. The results of the experiments give grounds to suppose the presence of interrelation between alpha-tocopherol and biosynthesis of ubiquinone and ubichromenol in the rat liver.

• Authors: L A Chernukhina, G V Donchenko, O M Zolotashko, L Iu Teplitskaia
• Journal: Ukrains'kyi biokhimichnyi zhurnal (Ukr Biokhim Zh) 1976 Mar-Apr Vol. 48 Issue 2 Pg. 206-10 ISSN: 0041-610X [Print] Ukraine
• Vernacular Title: Vplyv fenilalaninu ĭ al'fa-tokoferolu na obmin ubikhinonu ta ubikhromenolu v pechintsi E-avitaminoznykh shchuriv in vitro
• PMID: 941313 (Publication Type: Journal Article)

Chemical References

• Benzopyrans
• Carbon Radioisotopes
• Chromans
• Ubiquinone
• Vitamin E
• ubichromenol
• Phenylalanine

Topics

• Animals
• Benzopyrans (metabolism)
• Carbon Radioisotopes
• Chromans (metabolism)
• Dose-Response Relationship, Drug
• Drug Interactions
• In Vitro Techniques
• Liver (drug effects, metabolism)
• Phenylalanine (pharmacology)
• Rats
• Scintillation Counting
• Time Factors
• Ubiquinone (metabolism)
• Vitamin E (pharmacology)
• Vitamin E Deficiency (metabolism)