Immunization of cattle with a BHV1 vector vaccine or a DNA vaccine both coding for the G protein of BRSV.

Abstract

A gE-negative bovine herpesvirus 1 (BHV1) vector vaccine carrying a gene coding for the G protein of bovine respiratory syncytial virus (BRSV) (BHV1/BRSV-G) induced the same high degree of protection in calves against BRSV infection and BHV1 infection as a multivalent commercial vaccine. A DNA plasmid vaccine, carrying the same gene as the BHV1/BRSV-G vaccine, significantly reduced BRSV shedding after BRSV infection compared with that in control calves, but less well than the BHV1/BRSV-G vaccine. Flow cytometric analysis showed a significant relative increase of gamma/delta+ T cells in peripheral blood after BRSV challenge-infection of the calves of the control group but not in the vaccinated groups. These results indicate that the G protein of BRSV can induce significant protection against BRSV infection in cattle, and that the BHV1/BRSV-G vaccine protects effectively against a subsequent BRSV and BHV1 infection.

• Authors: R S Schrijver, J P Langedijk, G M Keil, W G Middel, M Maris-Veldhuis, J T Van Oirschot, F A Rijsewijk
• Journal: Vaccine (Vaccine) Vol. 15 Issue 17-18 Pg. 1908-16 (Dec 1997) ISSN: 0264-410X [Print] Netherlands
• PMID: 9413101 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• G protein, Bovine respiratory syncytial virus
• Vaccines, Combined
• Vaccines, DNA
• Viral Envelope Proteins
• Viral Vaccines

Topics

• Animals
• Antibodies, Viral (immunology)
• Cattle
• Cattle Diseases (immunology, prevention & control)
• Herpesviridae Infections (veterinary)
• Herpesvirus 1, Bovine (genetics, immunology)
• Respiratory Syncytial Virus Infections (immunology, veterinary)
• Respiratory Syncytial Virus, Bovine (genetics, immunology)
• T-Lymphocyte Subsets (immunology)
• Vaccination (veterinary)
• Vaccines, Combined (genetics, immunology, therapeutic use)
• Vaccines, DNA (genetics, therapeutic use)
• Viral Envelope Proteins (genetics, immunology)
• Viral Vaccines (genetics, immunology, therapeutic use)