Abstract | BACKGROUND: Nude mice with xenografted human tumors is the most exploited animal model used to elucidate the efficacy of experimental radioimmunolocalization and radioimmunotherapy. These animals accept transplants and are generally considered immunologically inert with regard to cell-mediated and humoral immune responses against the tumors. METHODS: Nude control mice and mice carrying human HeLa Hep-2 tumor xenografts were studied for appearance of endogenous antibodies following inoculation with tumor cells. The titers of these antibodies were investigated by isotype-specific enzyme-linked immunosorbent assay (ELISA) technologies, fluorescence-activated cell sorter analysis (FACS), BIAcore (Pharmacia Biosensor AB, Uppsala, Sweden) technology, and immunofluorescence. RESULTS: CONCLUSIONS: Nude mice bearing tumor xenografts produce significant amounts of antibodies against these two human tumor-derived antigens. These endogenous antibodies may influence targeting of radiolabeled antibodies. They also have the potential to interfere with the pharmacokinetics of labeled or nonlabeled idiotypic antibodies during experimental immunolocalization.
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Authors | T Stigbrand, A Ullén, P Sandström, S Rathsman, R Rossi Norrlund, L Arlestig, K Riklund Ahlström, S O Hietala |
Journal | Cancer
(Cancer)
Vol. 80
Issue 12 Suppl
Pg. 2404-10
(Dec 15 1997)
ISSN: 0008-543X [Print] United States |
PMID | 9406690
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Neoplasm
- Keratins
- Alkaline Phosphatase
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Topics |
- Alkaline Phosphatase
(immunology)
- Animals
- Antibodies, Neoplasm
(biosynthesis)
- Enzyme-Linked Immunosorbent Assay
- Female
- Fluorescent Antibody Technique
- Humans
- Keratins
(immunology)
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Neoplasm Transplantation
- Neoplasms, Experimental
(immunology)
- Transplantation, Heterologous
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