Amphistin, a new melanogenesis inhibitor, produced by an actinomycete.

Abstract

A new melanogenesis inhibitor, named amphistin, was isolated from the fermentation broth of an actinomycete strain KP-3052. Amphistin was purified from the culture filtrate by the combination of cation exchange, gel filtration, and aminosilyl silica gel chromatographic methods. The structure of amphistin was elucidated as gamma-(beta-histidinoalanino)homoalanine by NMR experiments including 1H-15N HMBC experiment and other spectroscopic analyses. Amphistin inhibited the melanogenesis of B16 melanoma cells at concentration of 6.8 microM.

• Authors: N Arai, K Shiomi, S Takamatsu, K Komiyama, M Shinose, Y Takahashi, Y Tanaka, Y Iwai, J R Liu, S Omura
• Journal: The Journal of antibiotics (J Antibiot (Tokyo)) Vol. 50 Issue 10 Pg. 808-14 (Oct 1997) ISSN: 0021-8820 [Print] England
• PMID: 9402984 (Publication Type: Journal Article)

Chemical References

• Aminobutyrates
• Imidazoles
• Melanins
• amphistin

Topics

• Actinomycetaceae (chemistry, metabolism)
• Aminobutyrates (chemistry, metabolism, pharmacology)
• Animals
• Bacteria (drug effects)
• Fermentation
• Fungi (drug effects)
• Imidazoles (chemistry, metabolism, pharmacology)
• Melanins (biosynthesis)
• Melanoma, Experimental
• Mice
• Microbial Sensitivity Tests
• Molecular Structure
• Tumor Cells, Cultured