Abstract |
A new melanogenesis inhibitor, named amphistin, was isolated from the fermentation broth of an actinomycete strain KP-3052. Amphistin was purified from the culture filtrate by the combination of cation exchange, gel filtration, and aminosilyl silica gel chromatographic methods. The structure of amphistin was elucidated as gamma-(beta-histidinoalanino) homoalanine by NMR experiments including 1H-15N HMBC experiment and other spectroscopic analyses. Amphistin inhibited the melanogenesis of B16 melanoma cells at concentration of 6.8 microM.
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Authors | N Arai, K Shiomi, S Takamatsu, K Komiyama, M Shinose, Y Takahashi, Y Tanaka, Y Iwai, J R Liu, S Omura |
Journal | The Journal of antibiotics
(J Antibiot (Tokyo))
Vol. 50
Issue 10
Pg. 808-14
(Oct 1997)
ISSN: 0021-8820 [Print] England |
PMID | 9402984
(Publication Type: Journal Article)
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Chemical References |
- Aminobutyrates
- Imidazoles
- Melanins
- amphistin
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Topics |
- Actinomycetaceae
(chemistry, metabolism)
- Aminobutyrates
(chemistry, metabolism, pharmacology)
- Animals
- Bacteria
(drug effects)
- Fermentation
- Fungi
(drug effects)
- Imidazoles
(chemistry, metabolism, pharmacology)
- Melanins
(biosynthesis)
- Melanoma, Experimental
- Mice
- Microbial Sensitivity Tests
- Molecular Structure
- Tumor Cells, Cultured
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