Abstract |
DNA synthesis activity was examined in fibroblasts and isolated nuclei derived from patients with nevoid basal cell carcinoma syndrome ( NBCCS) upon exposure to X-ray and ultraviolet (UV). The DNA synthesis activity in NBCCS fibroblasts increased after X-ray irradiation, i.e., to twice that on mock-irradiation, while it decreased in healthy donor-derived fibroblasts. The DNA synthesis activity in isolated nuclei of X-ray irradiated NBCCS fibroblasts also increased, i.e., more than twice that on mock-irradiated. In the experiments using synchronized cells, DNA synthesis activity showed the most marked increase when the fibroblasts at S phase were irradiated with X-rays. In contrast, UV-irradiated NBCCS fibroblasts showed no such increase in DNA synthesis. These results revealed that DNA synthesis is abnormally induced in X-ray irradiated NBCCS cells and that this abnormality might be related with the tendency of tumorigenesis in NBCCS patients after exposure to X-ray.
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Authors | K Fujii, N Suzuki, S Ishijima, K Kita, T Sonoda, M Dezawa, K Sugita, H Niimi |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 240
Issue 2
Pg. 269-72
(Nov 17 1997)
ISSN: 0006-291X [Print] United States |
PMID | 9388465
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Neoplasm
- Microfilament Proteins
- Proteins
- Tex9 protein, mouse
- Thymine Nucleotides
- thymidine 5'-triphosphate
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Topics |
- Ataxia Telangiectasia
- Basal Cell Nevus Syndrome
(metabolism, pathology)
- Cell Line
- Cell Nucleus
(metabolism, radiation effects)
- DNA Replication
(radiation effects)
- DNA, Neoplasm
(biosynthesis, radiation effects)
- Fibroblasts
- Humans
- Kinetics
- Microfilament Proteins
- Protein Biosynthesis
- Proteins
(chemistry)
- Skin Neoplasms
(metabolism, pathology)
- Thymine Nucleotides
(metabolism)
- Tumor Cells, Cultured
- Ultraviolet Rays
- X-Rays
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