Abstract |
Only small amounts of superoxide dismutase (SOD) are present in the extracellular space to scavenge excess amounts of superoxide anions (02-) released after traumatic brain injury (TBI). Experiments were performed in rats with cerebral contusion produced by weight-drop technique. We investigated the effects of exogenous lecithinized SOD (PC-SOD) on accumulation of 02- produced in our model, by measuring the level of SOD activity (using the NBT-reducing method) and the expression of copper, zinc-SOD (Cu, Zn-SOD) mRNA (by Northern blot analysis). As determined by tissue-specific gravity, administration of PC-SOD reduced brain edema in the periphery of the lesion 6 h after contusion. SOD activity increased in the peripheral region at 30 min after contusion, but returned to normal levels at 6 h after TBI. Administration of PC-SOD increased SOD activity up to 6 h after TBI. The expression of Cu, Zn-SOD mRNA increased in the core region, peripheral portion, and contralateral hemisphere up to 6 h after TBI, then was suppressed in all three regions by PC-SOD. Our results confirm the important role of 02- in the development of brain edema after TBI and indicate that PC-SOD diminishes brain edema through a protective effect against 02-.
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Authors | M Yunoki, M Kawauchi, N Ukita, Y Noguchi, S Nishio, Y Ono, S Asari, T Ohmoto, M Asanuma, N Ogawa |
Journal | Journal of neurotrauma
(J Neurotrauma)
Vol. 14
Issue 10
Pg. 739-46
(Oct 1997)
ISSN: 0897-7151 [Print] United States |
PMID | 9383092
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Phosphatidylcholines
- RNA, Messenger
- Superoxide Dismutase
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Topics |
- Animals
- Body Water
(metabolism)
- Brain Injuries
(metabolism)
- Cerebral Cortex
(drug effects, metabolism)
- Male
- Phosphatidylcholines
(pharmacology)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Superoxide Dismutase
(genetics, metabolism, pharmacology)
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