Immunization of cystic fibrosis patients with a Pseudomonas aeruginosa O-polysaccharide-toxin A conjugate vaccine.

• Abstract: Healthy, non-colonized cystic fibrosis (CF) patients (N = 26) were immunized with an octavalent Pseudomonas aeruginosa O-polysaccharide-toxin A conjugate vaccine. Vaccination was well tolerated and induced anti-lipopolysaccharide (LPS) antibodies of a high affinity capable of promoting the opsonophagocytic killing of P. aeruginosa by human peripheral lymphocytes. In contrast, anti-LPS antibodies acquired after natural infection possessed a very low affinity and were non-opsonic. To determine if immunization could prevent or delay infections due to P. aeruginosa, the infection rate among immunized patients was compared retrospectively to age and gender-matched controls. After 6 years of clinical follow-up, 15/20 (75%) of control and 8/23 (35%) of immunized subjects were classified as infected (p = 0.022). The persistence of high-affinity antibodies among immunized patients correlated with a significantly lower rate of infection after 4-6 years of observation. Infection of immunized patients was correlated with a dramatic decline in total antibody titer between year 2 and 3 of follow-up. Smooth, typeable strains of P. aeruginosa predominated among immunized patients. In contrast, rough, nontypeable strains were most frequently isolated from nonimmunized patients. Mucoid P. aeruginosa strains were isolated from 6 nonimmunized patients versus only I immunized subject.
• Authors: S J Cryz Jr, A Lang, A Rüdeberg, J Wedgwood, J U Que, E Fürer, U Schaad (Affiliation: Swiss Serum and Vaccine Institute, Berne, Switzerland.)
• Journal: Behring Institute Mitteilungen (Behring Inst Mitt) Issue 98 Pg. 345-9 (Feb 1997) ISSN: 0301-0457 [Print] GERMANY
• PMID: 9382759 (Publication Type: Clinical Trial, Comparative Study, Controlled Clinical Trial, Journal Article)

Chemical References

• Bacterial Toxins
• Bacterial Vaccines
• Exotoxins
• O Antigens
• Vaccines, Synthetic
• Virulence Factors
• ADP Ribose Transferases
• toxA protein, Pseudomonas aeruginosa

Topics

• ADP Ribose Transferases
• Adolescent
• Adult
• Bacterial Toxins
• Bacterial Vaccines
• Child
• Child, Preschool
• Cystic Fibrosis (complications, immunology, microbiology)
• Exotoxins (immunology)
• Humans
• Incidence
• Infant
• Lymphocytes (immunology, microbiology)
• O Antigens (immunology)
• Phagocytosis
• Pseudomonas Infections (epidemiology, immunology, prevention & control)
• Pseudomonas aeruginosa (immunology)
• Vaccines, Synthetic
• Virulence Factors