Abstract |
For the development of immunotherapy using MAGE peptides, the identification of additional tumor antigens is required. Because HLA-A24 is the most common allele in Japanese and is also frequently present in Caucasians, MAGE-3-encoded synthetic peptides with binding affinity for HLA-A24 were thus tested for the induction of specific CTLs from the peripheral blood mononuclear cells (PBMCs) of HLA-A24 healthy donors using a simplified method. By using a peptide with a sequence of IMPKAGLLI ( amino acid position in MAGE-3 195-203), the CTL responses could thus be induced from unseparated PBMCs by stimulation with freshly isolated, peptide-pulsed PBMCs as antigen-presenting cells (APCs) and by also using interleukin 7 and keyhole limpet hemocyanin for a primary culture. The induced CTLs could lyse HLA-A24 carcinoma cells expressing MAGE-3, as well as the peptide-pulsed target cells, in an HLA class-I restricted manner. The identification of the MAGE-3/ HLA-A24 peptide, IMPKAGLLI, may thus potentially offer the opportunities to design peptide-based immunotherapeutic approaches that might prove to be effective in treating patients with MAGE-3-positive malignant tumors.
|
Authors | F Tanaka, T Fujie, K Tahara, M Mori, K Takesako, A Sette, E Celis, T Akiyoshi |
Journal | Cancer research
(Cancer Res)
Vol. 57
Issue 20
Pg. 4465-8
(Oct 15 1997)
ISSN: 0008-5472 [Print] United States |
PMID | 9377553
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antigens, Neoplasm
- HLA-A Antigens
- HLA-A24 Antigen
- MAGEA3 protein, human
- Neoplasm Proteins
- Oligopeptides
|
Topics |
- Alleles
- Amino Acid Sequence
- Antigens, Neoplasm
(biosynthesis, immunology)
- B-Lymphocytes
- Binding Sites
- Cell Line
- Colonic Neoplasms
- Cytotoxicity, Immunologic
- Esophageal Neoplasms
- HLA-A Antigens
(immunology)
- HLA-A24 Antigen
- Humans
- Immunotherapy
(methods)
- Japan
- Leukemia
- Neoplasm Proteins
(biosynthesis, chemistry, immunology)
- Oligopeptides
(chemistry, immunology)
- Polymerase Chain Reaction
- Reference Values
- Stomach Neoplasms
- T-Lymphocytes, Cytotoxic
(immunology)
- Tumor Cells, Cultured
- White People
|