Primary sequence of the human, lysine-rich, ribosomal protein RPL38 and detection of an unusual RPL38 processed pseudogene in the promoter region of the type-1 angiotensin II receptor gene.

Abstract

We have isolated a cDNA clone encoding the human ribosomal protein L38 (HSRPL38). The longest ORF of the cDNA predicts a lysine-rich small polypeptide identical to the rat RPL38 protein (100% identity), and sharing a 84% of identity to the tomato RPL38 protein sequence. Northern blot analysis of a number of epithelial cell lines showed that the HSRPL38 is encoded by a mRNA ubiquitously expressed. Southern blot analysis of human genomic DNA suggested that the RPL38 does not constitute a multigene family but it is encoded by a reduced set of active genes, among which we have also found a RPL38 processed pseudogene located in the promoter region of the human type-1 angiotensin II receptor gene. This RPL38 pseudogene is very unusual among processed pseudogenes in that the poly A tail and the entire 5'-UTR of the original RPL38 mRNA were deleted during the retrotransposition process.

• Authors: L Espinosa, M Martín, A Nicolas, M Fabre, E Navarro
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1354 Issue 1 Pg. 58-64 (Oct 09 1997) ISSN: 0006-3002 [Print] Netherlands
• PMID: 9375793 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Complementary
• RPL38 protein, rat
• Receptors, Angiotensin
• Ribosomal Proteins
• RPL38 protein, human

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Cell Line
• DNA, Complementary
• Humans
• Molecular Sequence Data
• Open Reading Frames
• Promoter Regions, Genetic
• Pseudogenes
• Rats
• Receptors, Angiotensin (genetics)
• Ribosomal Proteins (genetics)
• Sequence Homology, Amino Acid
• Sequence Homology, Nucleic Acid