Abstract |
Chromosomal translocations involving band 5q31-35 occur in several hematologic disorders. A clone with a t(5; 14)(q33; q32) translocation appeared at the relapse phase in a patient with acute myelogenous leukemia who exhibited a sole chromosomal translocation, t(7; 11), at initial diagnosis. After the appearance of this clone, the leukemia progressed with marked eosinophilia, and combination chemotherapy was ineffective. Southern blot analysis showed a rearrangement of the platelet-derived growth factor receptor beta (PDGFRbeta) gene at 5q33 which was not observed at initial diagnosis. This translocation resulted in a chimeric transcript fusing the PDGFRbeta gene on 5q33 with a novel gene, CEV14, located at 14q32. Expression of the 5' region of the PDGFRbeta cDNA, upstream of the breakpoint, was not detected. However, the 3' region of PDGFRbeta, which was transcribed as part of the CEV14-PDGFRbeta fusion gene, was detected. A partial cDNA for a novel gene, CEV14, includes a leucine zipper motif and putative thyroid hormone receptor interacting domain and is expressed in a wide range of tissues. The expression of a CEV14-PDGFRbeta fusion gene in association with aggressive leukemia progression suggests that this protein has oncogenic potential.
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Authors | A Abe, N Emi, M Tanimoto, H Terasaki, T Marunouchi, H Saito |
Journal | Blood
(Blood)
Vol. 90
Issue 11
Pg. 4271-7
(Dec 01 1997)
ISSN: 0006-4971 [Print] United States |
PMID | 9373237
(Publication Type: Journal Article)
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Chemical References |
- CEV14-PDGFRbeta fusion protein, human
- Cytoskeletal Proteins
- Nuclear Proteins
- Oncogene Proteins, Fusion
- Platelet-Derived Growth Factor
- Proteins
- TRIP11 protein, human
- Receptor, Platelet-Derived Growth Factor beta
- Receptors, Platelet-Derived Growth Factor
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Topics |
- Amino Acid Sequence
- Base Sequence
- Chromosomes, Human, Pair 14
- Chromosomes, Human, Pair 5
- Clone Cells
(chemistry)
- Cytoskeletal Proteins
- Eosinophilia
(genetics, pathology)
- Humans
- In Situ Hybridization, Fluorescence
- Leukemia, Myeloid, Acute
(genetics, pathology)
- Molecular Sequence Data
- Nuclear Proteins
- Oncogene Proteins, Fusion
(chemistry, genetics)
- Platelet-Derived Growth Factor
- Polymerase Chain Reaction
- Proteins
(chemistry, genetics)
- Receptor, Platelet-Derived Growth Factor beta
- Receptors, Platelet-Derived Growth Factor
(genetics)
- Translocation, Genetic
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