Hemolysis of 40% hematocrit, Albunex-supplemented human erythrocytes by pulsed ultrasound: frequency, acoustic pressure and pulse length dependence.

Abstract

The dependence of hemolysis produced by pulsed ultrasound on ultrasound frequency, acoustic pressure and pulse length was explored. Human erythrocytes (40% hematocrit; in Albunex-supplemented autologous plasma) were exposed (60 s) to 20 or 200 microns pulses of ultrasound at frequencies of 1.02, 2.24 or 3.46 MHz and at peak negative pressures [P-] ranging from 0.0 to approximately 3.0 MPa in 0.5 MPa increments. The duty factor was 0.01. At each frequency, hemolysis increased with increasing acoustic pressure and depended weakly on pulse duration. At relatively high acoustic pressures, hemolysis depended strongly on ultrasound frequency; at lower pressures, the frequency dependence was weaker. The potential clinical significance of ultrasonic hemolysis is discussed.

• Authors: A A Brayman, P L Strickler, H Luan, S L Barned, C H Raeman, C Cox, M W Miller
• Journal: Ultrasound in medicine & biology (Ultrasound Med Biol) Vol. 23 Issue 8 Pg. 1237-50 ( 1997) ISSN: 0301-5629 [Print] England
• PMID: 9372572 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Albumins
• Albunex
• Contrast Media

Topics

• Albumins
• Contrast Media
• Erythrocytes
• Hematocrit
• Hemolysis
• Humans
• In Vitro Techniques
• Microspheres
• Ultrasonics (adverse effects)