Alteration of intracellular free calcium and acylphosphatase levels in differentiating SH-SY5Y neuroblastoma cells.

Abstract	Levels of acylphosphatase isoenzymes and free intracellular calcium have been investigated in cultured SH-SY5Y human neuroblastoma cells under stimulation with all-trans retinoic acid and phorbol-12-myristate-13-acetate. Under these conditions morphological and functional characteristics demonstrated the differentiation of SH-SY5Y cells towards neuronal phenotype. Retinoic acid treatment caused a progressive and synchronous increase of the organ common-type acylphosphatase and of free intracellular calcium but not of the muscle-type acylphosphatase. Phorbol-12-myristate-13-acetate treatment gave rise to a peak of the muscle-type acylphosphatase levels during the early differentiation stage whereas organ common-type isoenzyme and free calcium levels show a pattern similar to that observed in retinoic acid-treated cells. These evidences indicate that the two acylphosphatase isoenzymes play different roles in SH-SY5Y differentiation and that during this process the expression of organ common-type acylphosphatase increases in a synchronous way with intracellular free calcium concentration.
Authors	A Pieri, G Liguri, C Cecchi, D Degl'Innocenti, P Nassi, G Ramponi
Journal	Biochemistry and molecular biology international (Biochem Mol Biol Int) Vol. 43 Issue 3 Pg. 633-41 (Oct 1997) ISSN: 1039-9712 [Print] England
PMID	9352082 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Carcinogens Neoplasm Proteins Phorbol Esters Tretinoin Acid Anhydride Hydrolases acylphosphatase Calcium
Topics	Acid Anhydride Hydrolases (drug effects, metabolism) Calcium (metabolism) Carcinogens (pharmacology) Cell Differentiation (drug effects) Cell Division (drug effects) Humans Neoplasm Proteins (drug effects, metabolism) Neuroblastoma (metabolism, pathology) Phorbol Esters (pharmacology) Time Factors Tretinoin (pharmacology) Tumor Cells, Cultured (drug effects)

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