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Polymorphous low-grade adenocarcinoma: flow cytometric, p53, and PCNA analysis.

Abstract
Polymorphous low-grade adenocarcinoma of minor salivary glands (terminal duct carcinoma, lobular carcinoma) was first defined more than a decade ago. A 17% recurrence rate and a 9% metastasis rate have been reported. Fifteen formalin-fixed, paraffin-embedded archival cases were analyzed. Ploidy and proliferative activity were evaluated with flow cytometric analysis. Demonstration of an abnormal p53 gene product and proliferative cell nuclear antigen analyses were also performed with routine immunohistochemical procedures. The purpose of this investigation was to evaluate these parameters and determine if a correlation existed. Flow cytometry was performed on 10 cases; 3 showed an aneuploid cell line (mean, S-phase diploid tumor cells 5.9%; S-phase aneuploid 26.7%). Products of a mutation of the p53 tumor suppressor gene have been noted to accumulate in salivary gland tumors, both benign and malignant. Qualitative assessment revealed p53 positive staining in 4 of 15 tumors; positive cells comprised 5% to 10% of the tumor. The percentage of tumor cells positive for proliferative cell nuclear antigen staining ranged from 0.5% to 70%. There was no correlation between proliferative activity as determined by proliferative cell nuclear antigen when compared with results of flow cytometric analysis except for one case that exhibited p53 staining, a 26% proliferative cell nuclear antigen fraction, and a distinct aneuploid cell line.
AuthorsR D Kelsch, T Bhuiya, A Fuchs, P Gentile, M A Kahn, J E Fantasia
JournalOral surgery, oral medicine, oral pathology, oral radiology, and endodontics (Oral Surg Oral Med Oral Pathol Oral Radiol Endod) Vol. 84 Issue 4 Pg. 391-9 (Oct 1997) ISSN: 1079-2104 [Print] United States
PMID9347503 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • DNA, Neoplasm
  • Fixatives
  • Proliferating Cell Nuclear Antigen
  • Tumor Suppressor Protein p53
  • Formaldehyde
Topics
  • Adenocarcinoma (genetics, pathology, secondary)
  • Adult
  • Aged
  • Aneuploidy
  • Cell Division
  • DNA, Neoplasm (analysis)
  • Diploidy
  • Female
  • Fixatives
  • Flow Cytometry
  • Formaldehyde
  • Genes, p53 (genetics)
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Mutation (genetics)
  • Neoplasm Recurrence, Local (pathology)
  • Paraffin Embedding
  • Ploidies
  • Proliferating Cell Nuclear Antigen (analysis)
  • S Phase
  • Salivary Ducts (pathology)
  • Salivary Gland Neoplasms (genetics, pathology)
  • Salivary Glands, Minor (pathology)
  • Tissue Fixation
  • Tumor Suppressor Protein p53 (analysis)

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