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Determination of a new hypoxia selective agent from 2-quinoxalinecarbonitrile 1,4-di-N-oxides in plasma by high performance liquid chromatography.

Abstract
A high performance liquid chromatography (HPLC) method was developed and validated for the determination of 7-chloro-3-[[(N,N-dimethylamino)propyl]amino]-2-quinaxolinecarbonitri le 1,4-di-N-oxide (Q-85), a new hypoxia-selective agent, in plasma. The assay involves extraction into diethylether-dichloromethane (2:1) from plasma and subsequent analysis by reversed-phase HPLC with ultraviolet detection at 292 nm. Response was linear in the range of 0.4-20 micrograms/ml of plasma and the detection limit was 0.05 micrograms/ml. The extraction recovery was about 90%. The within and between day coefficients of variation did not exceed 6.1%. This HPLC assay has been applied to determine Q-85 in plasma samples taken during pharmacokinetic studies in mice.
AuthorsE Zamalloa, C Dios-Viéitez, E González-Peñas, A Monge, I Aldana
JournalArzneimittel-Forschung (Arzneimittelforschung) Vol. 47 Issue 9 Pg. 1044-6 (Sep 1997) ISSN: 0004-4172 [Print] Germany
PMID9342420 (Publication Type: Journal Article)
Chemical References
  • 7-chloro-3-(((N,N-dimethylamino)propyl)amino)-2-quinoxalinecarbonitrile-1,4-di-N-oxide
  • Quinoxalines
Topics
  • Animals
  • Calibration
  • Chromatography, High Pressure Liquid
  • Female
  • Hypoxia (metabolism)
  • Injections, Intravenous
  • Mice
  • Quinoxalines (administration & dosage, blood, pharmacokinetics)
  • Spectrophotometry, Ultraviolet

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