Abstract |
A high performance liquid chromatography (HPLC) method was developed and validated for the determination of 7-chloro-3-[[(N,N-dimethylamino)propyl]amino]-2-quinaxolinecarbonitri le 1,4-di-N-oxide (Q-85), a new hypoxia-selective agent, in plasma. The assay involves extraction into diethylether- dichloromethane (2:1) from plasma and subsequent analysis by reversed-phase HPLC with ultraviolet detection at 292 nm. Response was linear in the range of 0.4-20 micrograms/ml of plasma and the detection limit was 0.05 micrograms/ml. The extraction recovery was about 90%. The within and between day coefficients of variation did not exceed 6.1%. This HPLC assay has been applied to determine Q-85 in plasma samples taken during pharmacokinetic studies in mice.
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Authors | E Zamalloa, C Dios-Viéitez, E González-Peñas, A Monge, I Aldana |
Journal | Arzneimittel-Forschung
(Arzneimittelforschung)
Vol. 47
Issue 9
Pg. 1044-6
(Sep 1997)
ISSN: 0004-4172 [Print] Germany |
PMID | 9342420
(Publication Type: Journal Article)
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Chemical References |
- 7-chloro-3-(((N,N-dimethylamino)propyl)amino)-2-quinoxalinecarbonitrile-1,4-di-N-oxide
- Quinoxalines
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Topics |
- Animals
- Calibration
- Chromatography, High Pressure Liquid
- Female
- Hypoxia
(metabolism)
- Injections, Intravenous
- Mice
- Quinoxalines
(administration & dosage, blood, pharmacokinetics)
- Spectrophotometry, Ultraviolet
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