Inhibition of collagenases from mouse lung carcinoma cells by green tea catechins and black tea theaflavins.

Abstract

Theaflavin and theaflavin digallate, which are components of black tea were examined by in vitro invasion assay with mouse Lewis lung carcinoma LL2-Lu3 cells, which are highly metastatic. The compounds inhibited invasion by the tumor cells. Gelatin zymography showed that the cells secreted matrix metalloproteinases (MMPs), probably including MMP-2 and MMP-9, which may be involved in tumor cell invasion and metastasis. Theaflavin and theaflavin digallate also inhibited MMPs from the culture medium of these tumor cells, as did (-)-epigallocatechin gallate. These results suggest that theaflavin, theaflavin digallate, and (-)-epigallocatechin gallate inhibit tumor cell invasion by inhibiting type IV collagenases of the LL2-Lu3 cells.

• Authors: M Sazuka, H Imazawa, Y Shoji, T Mita, Y Hara, M Isemura
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 61 Issue 9 Pg. 1504-6 (Sep 1997) ISSN: 0916-8451 [Print] England
• PMID: 9339552 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Biflavonoids
• Free Radical Scavengers
• Matrix Metalloproteinase Inhibitors
• Tea
• theaflavin
• theaflavin digallate
• Gallic Acid
• Catechin
• epigallocatechin gallate

Topics

• Animals
• Antioxidants (pharmacology)
• Biflavonoids
• Carcinoma (enzymology)
• Catechin (analogs & derivatives, pharmacology)
• Free Radical Scavengers (pharmacology)
• Gallic Acid (analogs & derivatives, pharmacology)
• Lung Neoplasms (enzymology)
• Matrix Metalloproteinase Inhibitors
• Mice
• Tea (chemistry)
• Tumor Cells, Cultured