Abstract |
Bleomycin hydrolase (BH) is the only known eukaryotic enzyme that inactivates the widely used antineoplastic agent bleomycin (BLM) and is a primary candidate gene for protection against lethal BLM-induced pulmonary fibrosis and for BLM resistance in tumors. Human BH was found to exist as a single gene that was mapped to chromosome 17 using National Institute of General Medical Sciences human/rodent hybrid mapping panels and localized to 17q11.1-11.2 by linkage analysis using the Centre d'Etude du Polymorphisme Humain reference database. The human BH gene consisted of 11 exons ranging in size from 69-198 bp separated by introns of approximately 1 kb, reflecting the archetypal genomic structure of the cysteine protease family. A polymorphic site was identified in the eleventh exon at bp 1450 encoding either valine or isoleucine. These findings provide essential tools required to define the role of BH in BLM-induced pulmonary fibrosis and BLM resistance in tumors.
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Authors | S E Montoya, R E Ferrell, J S Lazo |
Journal | Cancer research
(Cancer Res)
Vol. 57
Issue 19
Pg. 4191-5
(Oct 01 1997)
ISSN: 0008-5472 [Print] United States |
PMID | 9331073
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Bleomycin
- Cysteine Endopeptidases
- bleomycin hydrolase
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Topics |
- Animals
- Base Sequence
- Bleomycin
(adverse effects)
- Chromosome Mapping
- Chromosomes, Human, Pair 17
(genetics)
- Cysteine Endopeptidases
(genetics)
- Disease Susceptibility
- Drug Resistance, Neoplasm
- Exons
(genetics)
- Genes
- Humans
- Hybrid Cells
- Molecular Sequence Data
- Polymorphism, Genetic
- Pulmonary Fibrosis
(chemically induced, genetics)
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