Reticalmin: a novel calcium/calmodulin-dependent protein kinase IV-like protein in rat retina.

Abstract

Western blot analysis of 100,000 g supernatant of rat retina using a polyclonal anti-Ca2+/ calmodulin-dependent protein kinase IV (CaM-kinase IV) antibody revealed an immunoreactive mass of 35 kDa, termed reticalmin. Lower amount of a isoform of CaM-kinase IV was also expressed in rat retina. Reticalmin did not react with anti-CaM-kinase IV C-terminal peptide antibody which recognized alpha and beta isoforms of CaM-kinase IV and calspermin. Immunohistochemically reticalmin was shown to be localized mainly in the outer segment of photo-receptor cells, and in dendrites of inner plexiform layers and may be in nuclei of ganglion cells and some inner nuclear layer cells.

• Authors: K Hirooka, M Tokuda, T Tsumura, B Y Ahmed, T Itano, H Matsui, R Konishi, E Hasegawa, S Okuno, T Kitani, H Fujisawa, O Hatase
• Journal: Vision research (Vision Res) Vol. 37 Issue 15 Pg. 2029-33 (Aug 1997) ISSN: 0042-6989 [Print] England
• PMID: 9327050 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Eye Proteins
• reticalmin
• Calcium-Calmodulin-Dependent Protein Kinases

Topics

• Animals
• Blotting, Western
• Calcium-Calmodulin-Dependent Protein Kinases (analysis)
• Eye Proteins (analysis)
• Immunohistochemistry
• Male
• Molecular Weight
• Rats
• Rats, Sprague-Dawley
• Retina (chemistry)