A severe, Th1-mediated experimental
colitis with similarities to
inflammatory bowel disease in humans can be induced by a single injection of
2,4,6-trinitrophenol (TNP)-substituted
protein plus adjuvant in IL-2-/- mice. To determine the early events involved in the pathogenesis of IL-2-/-
colitis, we compared the function of lamina propria (LP) T cells from IL-2-/- and IL-2+/+ mice subjected to disease-inducing (TNP-conjugated
keyhole limpet hemocyanin [
TNP-KLH]) and disease-inhibiting (anti-CD3) immunization protocols. We show that LP T cells in
TNP-KLH-immunized IL-2-/- mice fail to produce
TGF-beta early (day 2), whereas LP T cells in
TNP-KLH-immunized IL-2+/+ mice exhibit an approximately eightfold rise in
TGF-beta secretion. The critical importance of local
TGF-beta production was further substantiated by the following findings. 1) LP T cells from
TNP-KLH-immunized IL-2-/- mice administered anti-CD3 (i.p.) exhibit a significant rise in
TGF-beta, production but fail to produce IFN-gamma, and such mice do not develop
colitis. 2)
TNP-KLH-immunized IL-2-/- mice administered anti-CD3 and coadministered anti-
TGF-beta mAb again give rise to IFN-gamma-producing LP cells, and such mice develop
colitis. 3)
TNP-KLH-immunized IL-2+/+ mice administered anti-
TGF-beta mAb exhibit pockets of mononuclear cell infiltrates in the LP. These results indicate that the disposition of IL-2-/- mice to develop chronic colonic
inflammation is due to a Th1 cell response in the LP that is not appropriately counter-regulated by the production of the suppressor
cytokine,
TGF-beta.