Abstract |
N-terminal fragments of PTH and PTHrP, such as hPTH-(1-34) and hPTHrP-(1-34), are sufficiently similar with respect to amino acid sequence, location of functional domains, and higher order configuration to activate the same PTH/PTHrP receptor and the same two signal enzymes, adenylyl cyclase and phospholipase-Cbeta. Therefore, it was expected that hPTHrP-(1-31)NH2 would stimulate bone growth in ovariectomized rats as strongly as hPTH-(1-31)NH2. Like hPTH-(1-31)NH2, hPTHrP-(1-31)NH2 stimulated adenyly cyclase in ROS 17/2 osteosarcoma cells as strongly as the standard hPTH-(1-34) and like hPTH-(1-31)NH2, triggered a large drop in mean blood pressure when injected intravenously. Unlike hPTH-(1-31)NH2, however, hPTHrP-(1-31)NH2 could not stimulate trabecular growth in the distal femurs of young, sexually mature, ovariectomized rats.
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Authors | J F Whitfield, P Morley, G E Willick, V Ross, R Langille, S MacLean, J Barbier, R J Isaacs, L Ohannessian-Barry |
Journal | Calcified tissue international
(Calcif Tissue Int)
Vol. 61
Issue 4
Pg. 322-6
(Oct 1997)
ISSN: 0171-967X [Print] United States |
PMID | 9312203
(Publication Type: Journal Article)
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Chemical References |
- PTHLH protein, human
- Parathyroid Hormone
- Parathyroid Hormone-Related Protein
- Peptide Fragments
- Proteins
- parathyroid hormone (1-31)amide, human
- Adenylyl Cyclases
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Topics |
- Adenylyl Cyclases
(metabolism)
- Animals
- Blood Pressure
(drug effects)
- Bone Density
(drug effects)
- Bone Development
(drug effects)
- Bone Neoplasms
(enzymology, pathology)
- Disease Models, Animal
- Female
- Femur
(drug effects, physiology)
- Humans
- Injections, Intravenous
- Osteoporosis, Postmenopausal
(physiopathology)
- Osteosarcoma
(enzymology, pathology)
- Ovariectomy
- Parathyroid Hormone
(administration & dosage, pharmacology)
- Parathyroid Hormone-Related Protein
- Peptide Fragments
(administration & dosage, pharmacology)
- Proteins
(administration & dosage, pharmacology)
- Rats
- Rats, Sprague-Dawley
- Tumor Cells, Cultured
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