Analytical method for the determination of urinary 3-phenoxybenzoic acid in subjects occupationally exposed to pyrethroid insecticides.

Abstract

The determination of urinary 3-phenoxybenzoic acid enables exposure to pyrethroid insecticides to be evaluated. A method for the quantitative determination of this metabolite in urine is described. The compound and the internal standard (2-phenoxybenzoic acid) are derivatized with pentafluorobenzylbromide and transformed into pentafluorobenzyl esters, which are determined by gas chromatography with an intermediate polarity capillary column and an electron-capture detector. Before GC analysis, the urinary extracts are purified on LC-Si SPE columns. The proposed method has a detection limit of 0.5 microg/l and a mean recovery of 91.3%. The coefficient of variation of the analytical procedure, evaluated at a concentration of 24.96 microg/l, was 9.58%. Storage of the urine samples for 3 months at -18 degrees C did not lead to significant changes in the concentration of analyte. The method was tested analysing the urine of a farm worker with symptoms of pyrethroid poisoning, occupationally exposed to esfenvalerate.

• Authors: C Aprea, A Stridori, G Sciarra
• Journal: Journal of chromatography. B, Biomedical sciences and applications (J Chromatogr B Biomed Sci Appl) Vol. 695 Issue 2 Pg. 227-36 (Aug 01 1997) ISSN: 1387-2273 [Print] Netherlands
• PMID: 9300858 (Publication Type: Journal Article)

Chemical References

• Air Pollutants, Occupational
• Benzoates
• Insecticides
• Nitriles
• Pyrethrins
• 3-phenoxybenzoic acid
• fenvalerate

Topics

• Air Pollutants, Occupational (metabolism, poisoning)
• Benzoates (urine)
• Chromatography, Gas
• Humans
• Insecticides (metabolism, poisoning)
• Male
• Nitriles
• Pyrethrins (metabolism, poisoning)
• Sensitivity and Specificity