Abstract |
We have utilized 13C NMR spectroscopy to investigate glucose metabolism in the mouse insulinoma Beta TC3 cell line. Cells were cultured and examined both as monolayers and entrapped in alginate/poly-L-lysine/ alginate beads. Entrapped cultures were tested at 3 and 30 days post-entrapment. The purpose of this study was to assess whether the entrapped environment affects glucose metabolism and insulin secretion. Both monolayer and entrapped cultures were fed with 10 mM [1-(13)C]- glucose for 4 hrs. prior to extraction with perchloric acid. Our data revealed that beta TC3 cells possess a reduced tricarboxylic acid (TCA) activity in the entrapment cultures, and that they metabolized pyruvate primarily via pyruvate dehydrogenase regardless of the mode or age of the culture.
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Authors | I Constantinidis, N E Mukundan, M P Gamcsik, A Sambanis |
Journal | Cellular and molecular biology (Noisy-le-Grand, France)
(Cell Mol Biol (Noisy-le-grand))
Vol. 43
Issue 5
Pg. 721-9
(Jul 1997)
ISSN: 0145-5680 [Print] France |
PMID | 9298594
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Alginates
- Carbon Isotopes
- Hexuronic Acids
- Perchlorates
- Polylysine
- Lactic Acid
- Glucuronic Acid
- Glucose
- Eosine Yellowish-(YS)
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Topics |
- Alginates
(metabolism, pharmacology)
- Animals
- Carbon Isotopes
- Eosine Yellowish-(YS)
(chemistry)
- Glucose
(metabolism)
- Glucuronic Acid
- Glycolysis
- Hexuronic Acids
- Insulinoma
(drug therapy, metabolism)
- Lactic Acid
(metabolism)
- Magnetic Resonance Spectroscopy
(methods)
- Mice
- Pancreas, Artificial
- Perchlorates
(chemistry)
- Polylysine
(metabolism, pharmacology)
- Tumor Cells, Cultured
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