| Abstract | OBJECTIVE: To study at a molecular level the clonality of interleukin 2 (IL-2) expanded T cell lines derived from rheumatoid nodules. Such cell lines were reported in earlier studies with flow cytometry and antiidiotypic monoclonal antibodies (MAb) to be obligoclonal. METHODS: T cell lines were derived from rheumatoid nodules in 2 patients with rheumatoid arthritis (RA) and expanded in medium containing IL-2. Clonality was assessed by flow cytometry and T cell receptor (TCR) idiotype specific Mab and by polymerase chain reaction with primers for V alpha and V beta gene families. Sequence analysis was performed in selected cell lines. RESULTS: In one patient, one cell line was identified with marked overexpression of V alpha 2 cells. Eleven V alpha 2 CDR3 sequences were derived from this cell line: 8 of these clones had an identical CDR3 sequence and one other clone showed a related sequence. Five cell lines derived from a second patient displayed a marked clonal bias to V beta 8 cells. One cell line with strong V beta 8 expression was chosen for further sequence analysis. Twelve V beta 8 sequences were obtained; 11 showed identical CDR3 sequences. CONCLUSION: Molecular analysis of TCR rearrangements in IL-2 expanded T cell lines from rheumatoid nodules strongly suggests that in situ T cell activation is related to classical antigen induced immune activation. |
| Authors | F De Keyser, D Elewaut, J P Overmeer-Graus, P Van den Broek, A W Rijnders, E M Veys
(Affiliation: Department of Rheumatology, University Hospital Ghent, Belgium.)
|
| Journal | The Journal of rheumatology
(J Rheumatol)
Vol. 24
Issue 9
Pg. 1685-9
(Sep 1997)
ISSN: 0315-162X CANADA |
| PMID | 9292788
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- DNA Primers
- Interleukin-2
- Receptors, Antigen, T-Cell, alpha-beta
|
| Topics |
- Aged
- Arthritis, Rheumatoid
(complications)
- Cell Line
- Clone Cells
(immunology)
- DNA Primers
(chemistry)
- Flow Cytometry
- Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
(genetics)
- Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
(genetics)
- Humans
- Interleukin-2
(pharmacology)
- Lymphocyte Activation
(drug effects, genetics)
- Male
- Polymerase Chain Reaction
- Receptors, Antigen, T-Cell, alpha-beta
(drug effects, genetics, immunology)
- Rheumatoid Nodule
(immunology)
- T-Lymphocytes
(immunology)
|
