Molecular characterization of genes encoding a novel ABC transporter in Thermoanaerobacterium thermosulfurigenes EM1.

Abstract

The nucleotide sequence of two open reading frames (ORFs) from Thermoanaerobacterium thermosulfurigenes EM1 was determined that encode proteins with similarity to components of ATP-binding cassette (ABC) transport systems. Sequence analysis suggests that the deduced proteins AbcA and AbcB consist of an NH2-terminal membrane-spanning domain and a COOH-terminal ATP-binding domain. The deduced proteins AbcA and AbcB showed highest similarity to proteins of the MsbA subfamily of ABC transporters. AbcA and AbcB probably function as a heterodimer. An ORF predicted to encode the primary sigma factor SigA was identified downstream of abcB.

• Authors: M Matuschek, K Sahm, H Bahl
• Journal: Current microbiology (Curr Microbiol) Vol. 35 Issue 4 Pg. 237-9 (Oct 1997) ISSN: 0343-8651 [Print] United States
• PMID: 9290065 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP-Binding Cassette Transporters
• Bacterial Proteins

Topics

• ATP-Binding Cassette Transporters (chemistry, genetics)
• Amino Acid Sequence
• Bacteria, Anaerobic (genetics)
• Bacterial Proteins (chemistry, genetics)
• Chromosome Mapping
• Cloning, Molecular
• Genes, Bacterial
• Molecular Sequence Data
• Open Reading Frames
• Sequence Homology, Amino Acid