Abstract |
We investigated the effects of the biomaterials hydroxyapatite (HAP) and fluorapatite (FAP) on cultured human osteoarthritis type B synoviocytes by analyzing interleukin-1 alpha (IL-1 alpha) production and arachidonic acid metabolism via lipoxygenase pathways. A portion of opsonized particles was endocytosed and was found in numerous phagolysosomes in human synoviocyte cytoplasms. The present study demonstrates that HAP and FAP calcined at 700 degrees C induced a decrease in IL-1 alpha production but markedly decreased the synthesis of lipoxygenase products after 1-month incubation with the particles. This model will allow us to study the possible inflammatory response ( arachidonic acid metabolism, proinflammatory cytokines) that can be induced by any biomaterials used in orthopedics.
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Authors | B Liagre, J L Charissoux, M J Leboutet, D Bernache-Assollant, J L Beneytout |
Journal | Journal of biomedical materials research
(J Biomed Mater Res)
Vol. 38
Issue 3
Pg. 243-56
( 1997)
ISSN: 0021-9304 [Print] United States |
PMID | 9283970
(Publication Type: Journal Article)
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Chemical References |
- Apatites
- Biocompatible Materials
- Culture Media, Conditioned
- Interleukin-1
- Durapatite
- Lipoxygenase
- fluorapatite
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Topics |
- Apatites
(chemistry, metabolism)
- Biocompatible Materials
(chemistry, metabolism)
- Cells, Cultured
- Culture Media, Conditioned
- Durapatite
(chemistry, metabolism)
- Fluorescent Antibody Technique, Indirect
- Humans
- Interleukin-1
(biosynthesis, metabolism)
- Lipoxygenase
(biosynthesis, metabolism)
- Microscopy, Electron, Scanning
- Osteoarthritis
(enzymology, metabolism, pathology)
- Synovial Membrane
(enzymology, metabolism, pathology)
- X-Ray Diffraction
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