Involvement of interleukin-1 beta, nerve growth factor and prostaglandin E2 in endotoxin-induced localized inflammatory hyperalgesia.

Abstract

1. Intraplantar endotoxin (ET) injection (1.25 micrograms) into the hind paw of rats resulted in a localized inflammatory hyperalgesia, as assessed by paw pressure (PP), paw immersion (PI), tail flick (TF) and hot plate (HP) tests. 2. ET injection resulted in a significant elevation in the levels of interleukin-1 beta (IL-1 beta) and nerve growth factor (NGF) in the injected foot as compared with the non-injected foot. This increase was attenuated by intraperitoneal injections of dexamethasone (200 and 400 micrograms kg-1) and to a lesser extent by indomethacin (2 and 8 mg kg-1). 3. The tripeptide Lys-D-Pro-Val, which is known to antagonize IL-1 beta and prostaglandin E2 (PGE2) reversed mechanical hyperalgesia, as assessed by the PP test, and reduced significantly thermal hyperalgesia, as assessed by the HP and TF tests. 4. IL-1ra reversed both mechanical (PP) and thermal (PI) nociceptive thresholds tested on the injected leg and significantly reduced thermal hyperalgesia, as assessed by the HP and TF tests. 5. A sheep, anti-mouse NGF antiserum reversed mechanical hyperalgesia (PP test) but had little or no effect on thermal hyperalgesia (PI, HP and TF tests). 6. Our results indicate the importance of IL-1 beta, NGF and prostaglandin E2 (PGE2) in the development of ET induced hyperalgesia and the possible existence of different mechanisms underlying thermal and mechanical as well as central and peripheral hyperalgesia.

• Authors: B Safieh-Garabedian, S A Kanaan, J J Haddad, P A Jaoude, S J Jabbur, N E Saadé
• Journal: British journal of pharmacology (Br J Pharmacol) Vol. 121 Issue 8 Pg. 1619-26 (Aug 1997) ISSN: 0007-1188 [Print] England
• PMID: 9283695 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Endotoxins
• Il1rn protein, mouse
• Interleukin 1 Receptor Antagonist Protein
• Interleukin-1
• Nerve Growth Factors
• Sialoglycoproteins
• Dexamethasone
• Dinoprostone

Topics

• Animals
• Dexamethasone (pharmacology)
• Dinoprostone (physiology)
• Endotoxins (toxicity)
• Hyperalgesia (etiology)
• Interleukin 1 Receptor Antagonist Protein
• Interleukin-1 (physiology)
• Male
• Nerve Growth Factors (physiology)
• Rats
• Rats, Sprague-Dawley
• Sialoglycoproteins (pharmacology)