Abstract | PURPOSE: METHODS:
Messenger RNA from a specimen of human aortic aneurysmal adventitia was reverse-transcribed for insertion into the phagemid Uni Zap XR (Stratagene). A strain of Escherichia coli, engineered for expression (XL 1-Blue MFR', Stratagene), was transfected, and rabbit antihuman vitronectin antibody as used to identify positive clones. Sequencing of the positive clones was performed by the Core Laboratories at Columbia University. RESULTS: CONCLUSIONS:
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Authors | H Hirose, K J Ozsvath, S Xia, M D Tilson |
Journal | Journal of vascular surgery
(J Vasc Surg)
Vol. 26
Issue 2
Pg. 313-8
(Aug 1997)
ISSN: 0741-5214 [Print] United States |
PMID | 9279320
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Contractile Proteins
- DNA, Complementary
- Extracellular Matrix Proteins
- Immunoglobulin G
- RNA Splicing Factors
- RNA, Messenger
- microfibrillar protein
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Topics |
- Amino Acid Sequence
- Aortic Aneurysm, Abdominal
(genetics)
- Cloning, Molecular
- Contractile Proteins
(genetics)
- DNA, Complementary
(chemistry, genetics)
- Elastic Tissue
(metabolism)
- Extracellular Matrix Proteins
- Gene Expression Regulation
- Humans
- Immunoglobulin G
(immunology)
- Molecular Sequence Data
- RNA Splicing Factors
- RNA, Messenger
(genetics)
- Sequence Alignment
- Sequence Homology, Nucleic Acid
- Transcription, Genetic
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