Domon L, an immunopotentiating agent, acts as an NF-kappaB activator.

Abstract

Domon L, a heat-treated component of gram-negative bacterium Achromobacter stenohalis, is used for the treatment of infectious diseases of animals. Here, we investigated the immunopotentiating potential of Domon L. In vitro studies showed that Domon L enhanced nitric oxide (NO) formation from murine macrophage RAW264.7 cells in concert with interferon (IFN)-gamma. The effect of Domon L on NF-kappaB activation was investigated, in order to understand the molecular mechanisms of enhanced NO formation by Domon L. Domon L induced translocation of NF-kappaB to the nucleus in RAW264.7 cells. Induction of NF-kappaB dependent gene expression by Domon L was further confirmed using a transfectant containing an NF-kappaB-luciferase reporter gene. In vivo injection of Domon L elevated both serum IL-6 and mucoprotein, whose gene expression is partly under the control of NF-kappaB. The spleen cells of rats treated with Domon L produced much more NO when stimulated with LPS + IFN gamma than spleen cells of untreated rats. These results suggest that Domon L acts as an anti-infectious agent via NF-kappaB activation.

• Authors: S Tanaka, H Sakurai, M Sugiura, K Kawashima, T Sugita
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 59 Issue 7 Pg. 575-9 (Jul 1997) ISSN: 0916-7250 [Print] Japan
• PMID: 9271453 (Publication Type: Journal Article)

Chemical References

• Adjuvants, Immunologic
• Lipopolysaccharides
• NF-kappa B
• Nitrites
• Oligodeoxyribonucleotides
• Recombinant Fusion Proteins
• Nitric Oxide
• Interferon-gamma

Topics

• Achromobacter
• Adjuvants, Immunologic (pharmacology)
• Alcaligenes
• Animals
• Base Sequence
• Binding Sites
• Cell Line
• Female
• Interferon-gamma (pharmacology)
• Lipopolysaccharides (pharmacology)
• Macrophages (drug effects, physiology)
• Mice
• NF-kappa B (biosynthesis, metabolism)
• Nitric Oxide (biosynthesis)
• Nitrites (metabolism)
• Oligodeoxyribonucleotides (chemistry, metabolism)
• Rats
• Rats, Inbred F344
• Recombinant Fusion Proteins (biosynthesis)
• Transfection