The significance of Ras guanine nucleotide exchange factor, son of sevenless protein, in renal cell carcinoma cell lines.

Abstract

PURPOSE:

The aim of the present study is to clarify the significance of the Ras guanine-nucleotide exchange reaction in the proliferation of human renal cell carcinoma cell lines.

MATERIALS AND METHODS:

We examined the expression of human son of sevenless-1 (hSos-1) protein and the epidermal growth factor (EGF) receptor in human renal cell carcinoma cell lines by Western blot analysis. Additionally, a dominant negative H-ras mutant, N116Y, which is known to inhibit the Ras guanine-nucleotide exchange reaction, was transfected into these cell lines by lipofection.

RESULTS:

Human renal cell carcinoma cell lines expressed much higher amounts of the EGF receptor and hSos-1 protein than normal kidney tissue. Moreover, the N116Y ras mutant could strongly suppress cellular proliferation in these cell lines.

CONCLUSIONS:

Augmentation of the Ras guanine-nucleotide exchange reaction might be essential to the proliferation of human renal cell carcinoma cells.

• Authors: N Shinohara, Y Ogiso, M Tanaka, A Sazawa, T Harabayashi, T Koyanagi
• Journal: The Journal of urology (J Urol) Vol. 158 Issue 3 Pt 1 Pg. 908-11 (Sep 1997) ISSN: 0022-5347 [Print] United States
• PMID: 9258117 (Publication Type: Journal Article)

Chemical References

• Eukaryotic Initiation Factor-2
• Guanine Nucleotide Exchange Factors
• Proteins
• ras Guanine Nucleotide Exchange Factors
• ErbB Receptors

Topics

• Carcinoma, Renal Cell (genetics, pathology)
• Cell Division
• ErbB Receptors (genetics)
• Eukaryotic Initiation Factor-2 (genetics)
• Gene Expression Regulation, Neoplastic
• Genes, ras (genetics)
• Guanine Nucleotide Exchange Factors
• Humans
• Kidney Neoplasms (genetics, pathology)
• Mutation
• Proteins (genetics)
• Tumor Cells, Cultured
• ras Guanine Nucleotide Exchange Factors