Comparative sequence analysis of ribonucleases HII, III, II PH and D.

Abstract

Escherichia coli ribonucleases (RNases) HII, III, II, PH and D have been used to characterise new and known viral, bacterial, archaeal and eucaryotic sequences similar to these endo- (HII and III) and exoribonucleases (II, PH and D). Statistical models, hidden Markov models (HMMs), were created for the RNase HII, III, II and PH and D families as well as a double-stranded RNA binding domain present in RNase III. Results suggest that the RNase D family, which includes Werner syndrome protein and the 100 kDa antigenic component of the human polymyositis scleroderma (PMSCL) autoantigen, is a 3'-->5' exoribonuclease structurally and functionally related to the 3'-->5' exodeoxyribonuclease domain of DNA polymerases. Polynucleotide phosphorylases and the RNase PH family, which includes the 75 kDa PMSCL autoantigen, possess a common domain suggesting similar structures and mechanisms of action for these 3'-->5' phosphorolytic enzymes. Examination of HMM-generated multiple sequences alignments for each family suggest amino acids that may be important for their structure, substrate binding and/or catalysis.

• Authors: I S Mian
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 25 Issue 16 Pg. 3187-95 (Aug 15 1997) ISSN: 0305-1048 [Print] England
• PMID: 9241229 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Escherichia coli Proteins
• RNA-Binding Proteins
• ribonuclease PH
• Endoribonucleases
• Exoribonucleases
• exoribonuclease II
• ribonuclease HII
• Ribonuclease III
• ribonuclease III, E coli
• Ribonuclease H

Topics

• Amino Acid Sequence
• Endoribonucleases (genetics)
• Escherichia coli (enzymology, genetics)
• Escherichia coli Proteins
• Exoribonucleases (genetics)
• Genes, Bacterial
• Genes, Fungal
• Humans
• Molecular Sequence Data
• RNA-Binding Proteins (genetics)
• Ribonuclease H (genetics)
• Ribonuclease III
• Sequence Alignment
• Sequence Analysis