Studies on Agrobacterium tumefaciens. VIII. Avirulence induced by temperature and ethidium bromide.

When tumorigenic strains of Agrobacterium tumefaciens were subcultured at temperatures between 31.5 and 37 degrees C or in broth containing ethidium bromide, they lost their capacity to induce tumors in tomato plants. The sensitivities of curing virulence (tumorigenicity) depended on the density of the population of cells, fewer cells (100/ml) being more sensitive to curing than higher densities (10(6)/ml). The loss of virulence need not require the total loss of the virulence-specifying plasmid, but may result from a loss of a small segment of that plasmid. Virulent strains made avirulent by temperature or ethidium bromide treatment still harbor a large plasmid of 70-80 megadaltons size compared with the 100- to 120-megadalton plasmid in the untreated strains.
AuthorsB C Lin, C I Kado
JournalCanadian journal of microbiology (Can J Microbiol) Vol. 23 Issue 11 Pg. 1554-61 (Nov 1977) ISSN: 0008-4166 [Print] CANADA
PMID922605 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Ethidium
  • Ethidium (pharmacology)
  • Plant Diseases
  • Plasmids (drug effects)
  • Rhizobium (drug effects, pathogenicity)
  • Temperature

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: