[3H]raclopride binding to brain tissue from subjects with schizophrenia: methodological aspects.

Abstract

The binding of [3H]raclopride to particulate membrane and frozen sections (with quantitative autoradiography) from the caudate-putamen, obtained at autopsy from schizophrenic and non-schizophrenic subjects, was measured. The affinity of [3H]raclopride to particulate membrane was significantly decreased in the schizophrenic compared to non-schizophrenic subjects. The density of [3H]raclopride binding to tissue from subjects with schizophrenia was increased, unchanged or decreased depending on the methodology used. Finally, there was an age-dependent decrease in [3H]raclopride binding in the frozen sections from the caudate-putamen of the non-schizophrenic subjects. This age-dependent decrease was not apparent using particulate membrane from schizophrenic or non-schizophrenic subjects or tissue sections from the schizophrenic subjects. We conclude that the binding of [3H]raclopride is dependent on methodology and therefore data from in vitro and in vivo studies using this drug should be interpreted with caution.

• Authors: B Dean, G Pavey, K Opeskin
• Journal: Neuropharmacology (Neuropharmacology) Vol. 36 Issue 6 Pg. 779-86 (Jun 1997) ISSN: 0028-3908 [Print] England
• PMID: 9225305 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dopamine Antagonists
• Receptors, Dopamine D2
• Salicylamides
• Raclopride

Topics

• Adult
• Aged
• Autoradiography
• Brain (metabolism)
• Caudate Nucleus (metabolism)
• Cell Membrane (metabolism)
• Dopamine Antagonists (metabolism)
• Female
• Humans
• In Vitro Techniques
• Male
• Middle Aged
• Protein Binding
• Putamen (metabolism)
• Raclopride
• Receptors, Dopamine D2 (metabolism)
• Salicylamides (metabolism)
• Schizophrenia (metabolism)