Specificity of human cytotoxic T lymphocytes induced by a human papillomavirus type 16 E7-derived peptide.

Abstract

In order to establish tumour-specific cytotoxic T lymphocyte (CTL) cell lines, T cells from a human papillomavirus (HPV) type 16-positive patient with a cervical carcinoma in situ and from a healthy volunteer were stimulated in vitro with autologous dendritic cells loaded with peptides derived from the viral transforming proteins E6 and E7 and corresponding to potential HLA-A*0201-restricted T cell epitopes. From each donor a small number of low-affinity CTL lines against the peptide E7/86-93 was obtained, which specifically lysed HLA-A*0201-expressing B-lymphocytes (cell line 721) loaded with this peptide. Cytotoxicity was also observed against two HLA-A*0201-E7-positive epithelial cell lines, the cervical carcinoma cell line CaSki and the HPV-16-immortalized foreskin-keratinocyte cell line HPK IA. However, since none of the CTL recognized both cell lines, and E7-expressing 721 transfectants were never lysed, it was concluded that the reactivity against CaSki and HPK IA cells was due to cross-reactivity on allogeneic HLA molecules rather than to E7 recognition, which emphasizes that the specificity of tumour cell lysis by peptide-induced CTL has to be interpreted with caution.

• Authors: I Jochmus, W Osen, A Altmann, G Buck, B Hofmann, A Schneider, L Gissmann, H G Rammensee
• Journal: The Journal of general virology (J Gen Virol) Vol. 78 ( Pt 7) Pg. 1689-95 (Jul 1997) ISSN: 0022-1317 [Print] England
• PMID: 9225046 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HLA-A2 Antigen
• Oncogene Proteins, Viral
• Papillomavirus E7 Proteins
• Peptides
• oncogene protein E7, Human papillomavirus type 16

Topics

• Carcinoma in Situ (immunology)
• Cell Line
• Cytotoxicity Tests, Immunologic
• Female
• HLA-A2 Antigen (immunology)
• Humans
• Oncogene Proteins, Viral (genetics, immunology)
• Papillomaviridae (immunology)
• Papillomavirus E7 Proteins
• Papillomavirus Infections (immunology, virology)
• Peptides (chemical synthesis, genetics, immunology)
• T-Lymphocytes, Cytotoxic (cytology, immunology)
• Tumor Cells, Cultured
• Tumor Virus Infections (immunology, virology)
• Uterine Cervical Neoplasms (immunology)