Ribose 1-phosphate metabolism in Ehrlich ascites tumor cells in vitro.

Abstract

Ribose 1-phosphate concentrations have been measured in tumor cells incubated with purine and pyrimidine nucleosides and with glucose. Highest concentrations (0.15 to 0.2 mumol/ml of cells) were attained in cells incubated with inosine. Although uridine was cleaved at approximately the same rate as inosine, as judged by lactate accumulation, concentrations of ribose 1-phosphate that accumulated were only approximately 0.06 mumol/ml. Ribose 1-phosphate accumulation in tumor cells incubated with inosine was dependent on the phosphate concentration of the medium up to at least 25 mM. Ribose 1-phosphate formed from inosine was readily converted both to phosphoribosyl pyrophosphate and to lactate.

• Authors: J Barankiewicz, J F Henderson
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 479 Issue 4 Pg. 371-7 (Dec 14 1977) ISSN: 0006-3002 [Print] Netherlands
• PMID: 922006 (Publication Type: Journal Article)

Chemical References

• Pentosephosphates
• Purine Nucleosides
• Pyrimidine Nucleosides
• Ribosemonophosphates
• Glucose

Topics

• Animals
• Biological Transport
• Carcinoma, Ehrlich Tumor (metabolism)
• Glucose (pharmacology)
• Kinetics
• Mice
• Pentosephosphates (metabolism)
• Purine Nucleosides (pharmacology)
• Pyrimidine Nucleosides (pharmacology)
• Ribosemonophosphates (metabolism)