Abstract | OBJECTIVE: METHODS:
Phorbol myristate acetate (PMA)-induced differentiation of monocytes into multinucleated giant cells was used as an in vitro model to study the effects of adenosine on nodulosis. RESULTS: MTX at 200-2,000 nM or the adenosine A1 agonist N5-cyclopentyl adenosine (CPA) (10(-12) to 10(-9) M) or the A2 antagonist 3,7-dimethyl-1-propargylxanthine markedly enhanced giant cell formation, whereas the adenosine A1 antagonist 8-cyclopentyl-dipropylxanthine completely reversed these effects. PMA, CPA, and MTX induced adenosine release by cultured monocytes at concentrations consistent with those associated with predominantly A1 effects. Furthermore, surface expression of A1 receptors was found to remain unchanged on the differentiating cells throughout the culture period. CONCLUSION:
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Authors | J T Merrill, C Shen, D Schreibman, D Coffey, O Zakharenko, R Fisher, R G Lahita, J Salmon, B N Cronstein |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 40
Issue 7
Pg. 1308-15
(Jul 1997)
ISSN: 0004-3591 [Print] United States |
PMID | 9214432
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antirheumatic Agents
- Receptors, Purinergic P1
- Xanthines
- N(6)-cyclopentyladenosine
- 3,7-dimethyl-1-propargylxanthine
- 1,3-dipropyl-8-cyclopentylxanthine
- Adenosine
- Tetradecanoylphorbol Acetate
- Theobromine
- Methotrexate
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Topics |
- Adenosine
(analogs & derivatives, antagonists & inhibitors, pharmacology)
- Antirheumatic Agents
(adverse effects)
- Arthritis, Rheumatoid
(pathology)
- Cells, Cultured
- Humans
- Methotrexate
(adverse effects)
- Monocytes
(drug effects, pathology)
- Receptors, Purinergic P1
(physiology)
- Rheumatoid Nodule
(pathology)
- Tetradecanoylphorbol Acetate
(pharmacology)
- Theobromine
(analogs & derivatives, pharmacology)
- Xanthines
(pharmacology)
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