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Truncated-gene reporter system for studying the regulation of manganese peroxidase expression.

Abstract
The expression of manganese peroxidase (MnP) in nitrogen-limited cultures of Phanerochaete chrysosporium is regulated by Mn, heat shock (HS), and H2O2 at the level of gene transcription. We have constructed a homologous gene reporter system to further examine the regulation of two mnp genes, mnp1 and mnp2, encoding individual MnP isozymes. Internal deletions of 234 and 359 bp were made within the coding regions of the mnp1 and mnp2 genes, respectively. The truncated mnp genes were subcloned into the shuttle vector pOGI18, which includes the Schizophylum commune ade5 gene as a selectable marker, and transformed into a P. chrysosporium Ade1 auxotrophic mutant. Northern-blot analysis of purified Ade+ transformants demonstrated that both of the truncated mnp genes were regulated in a manner similar to the endogenous mnp genes with respect to nitrogen limitation and induction by Mn, HS, and H2O2.
AuthorsJ M Gettemy, D Li, M Alic, M H Gold
JournalCurrent genetics (Curr Genet) Vol. 31 Issue 6 Pg. 519-24 (Jun 1997) ISSN: 0172-8083 [Print] United States
PMID9211796 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Isoenzymes
  • Recombinant Proteins
  • Manganese
  • Hydrogen Peroxide
  • Peroxidases
  • manganese peroxidase
  • Nitrogen
  • Hydrogen Sulfide
Topics
  • Basidiomycota (genetics, metabolism)
  • Enzyme Activation
  • Gene Expression Regulation, Fungal
  • Genes, Reporter
  • Hot Temperature
  • Hydrogen Peroxide (metabolism)
  • Hydrogen Sulfide (metabolism)
  • Isoenzymes
  • Manganese (metabolism, pharmacology)
  • Nitrogen (metabolism)
  • Peroxidases (drug effects, genetics, metabolism)
  • Recombinant Proteins (genetics, metabolism)

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