Immune response to glycated and oxidized LDL in IDDM patients with and without renal disease.

Abstract

OBJECTIVE:

To study autoantibodies to oxidized and glycated LDL in IDDM patients with and without diabetic nephropathy and in nephropathy-related macroangiopathy

RESEARCH DESIGN AND METHODS:

The study included 101 IDDM patients with a long duration of diabetes and 54 healthy subjects. Patients were divided into two groups according to their median urinary albumin excretion rate (AER); the normoalbuminuric group had AER <20 microg/min and the albuminuric group >200 microg/min. The groups were matched for age and BMI, and the two diabetic groups were matched for duration of diabetes and glycemic control. Antibodies against oxidized LDL (using malondialdehyde-modified LDL as the antigen) and against glycated LDL were measured by enzyme-linked immunosorbent assay (ELISA).

RESULTS:

The mean antibody levels against glycated LDL were higher in IDDM patients (0.305 +/- 0.399) than in healthy subjects (0.166 +/- 0.22 optical density [OD]; P = 0.019), but levels did not differ significantly between normoalbuminuric and albuminuric IDDM patients (0.258 +/- 0.354 vs. 0.388 +/- 0.459, respectively). Among the three groups, antibody levels to oxidized LDL did not differ. IDDM patients showed an inverse correlation between antibodies to oxidized LDL and HbA1 (r = -0.211, P = 0.04). The antibody levels to glycated and oxidized LDL did not differ among albuminuric IDDM patients with or without clinical macroangiopathy.

CONCLUSIONS:

Antibodies to glycated and oxidized LDL do not seem to associate with diabetic nephropathy or nephropathy-related macroangiopathy.

• Authors: E Korpinen, P H Groop, H K Akerblom, O Vaarala
• Journal: Diabetes care (Diabetes Care) Vol. 20 Issue 7 Pg. 1168-71 (Jul 1997) ISSN: 0149-5992 [Print] United States
• PMID: 9203457 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Autoantibodies
• Glycated Hemoglobin A
• Glycation End Products, Advanced
• Lipoproteins, LDL
• glycated lipoproteins, LDL
• oxidized low density lipoprotein

Topics

• Adult
• Albuminuria (blood, physiopathology)
• Autoantibodies (analysis)
• Cohort Studies
• Diabetes Mellitus, Type 1 (complications, immunology)
• Diabetic Angiopathies (blood, immunology)
• Diabetic Nephropathies (complications, immunology)
• Enzyme-Linked Immunosorbent Assay
• Glycated Hemoglobin (analysis)
• Glycation End Products, Advanced
• Glycosylation
• Humans
• Lipoproteins, LDL (analysis, immunology)
• Middle Aged
• Oxidation-Reduction
• Reference Values