Abstract | OBJECTIVE: DESIGN: Prospective, experimental study. SETTING: Large animal research laboratory at a university medical center. INTERVENTIONS: Sheep (n = 23) were surgically prepared for chronic study. After a 5-day recovery period, all animals received a continuous infusion of live Pseudomonas aeruginosa (2.5 x 10(6) colony-forming units/min) for the next 48 hrs. After 24 hrs of sepsis, the animals were divided into four groups: a) six sheep were used as controls and received a bolus of 200-mL vehicle; b) three sheep received a bolus of 50 mg/kg hemoglobin; c) six sheep received 100 mg/kg of hemoglobin; d) six sheep received 200 mg/kg of hemoglobin. MEASUREMENTS AND MAIN RESULTS: All animals that survived the first 24 hrs of sepsis (n = 21) developed a hyperdynamic circulation. All three doses of hemoglobin reversed this hyperdynamic state by increasing mean arterial pressure and systemic vascular resistance while decreasing cardiac index. Pulmonary arterial pressure increased after hemoglobin infusion. Increased pulmonary arterial pressure did not affect arterial oxygen saturation nor result in the development of pulmonary edema. Infusion of hemoglobin also caused a 30-fold increase in endothelin-1 plasma concentrations and significantly decreased nitrate and nitrite plasma concentrations. CONCLUSIONS:
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Authors | H G Bone, P J Schenarts, M Booke, R McGuire, D Harper, L D Traber, D L Traber |
Journal | Critical care medicine
(Crit Care Med)
Vol. 25
Issue 6
Pg. 1010-8
(Jun 1997)
ISSN: 0090-3493 [Print] United States |
PMID | 9201055
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Substitutes
- Endothelin-1
- Hemoglobins
- Nitrates
- Nitrites
- pyridoxalated-hemoglobin-polyethylene glycol conjugate
- Polyethylene Glycols
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Topics |
- Animals
- Blood Circulation
(drug effects)
- Blood Pressure
(drug effects)
- Blood Substitutes
(pharmacology)
- Endothelin-1
(blood)
- Female
- Hemoglobins
(administration & dosage, pharmacology)
- Nitrates
(blood)
- Nitrites
(blood)
- Polyethylene Glycols
(pharmacology)
- Prospective Studies
- Sepsis
(physiopathology)
- Sheep
- Vascular Resistance
(drug effects)
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