Abstract |
Analysis by electrophoretic mobility shift assays (EMSA) of the different proteins associated with the kappaB sequence of the interleukin-6 (IL-6) promoter (IL6-kappaB) allowed us to detect a specific complex formed with the recombination signal sequence binding protein Jkappa (RBP-Jkappa). Single-base exchanges within the oligonucleotide sequence defined the critical base pairs involved in the interaction between RBP-Jkappa and the IL6-kappaB motif. Binding analysis suggests that the amount of RBP-Jkappa protein present in the nucleus is severalfold higher than the total amount of inducible NF-kappaB complexes but that the latter bind DNA with a 10-fold-higher affinity. A reporter gene study was performed to determine the functional implication of this binding; we found that the constitutive occupancy of the IL6-kappaB site by the RBP-Jkappa protein was responsible for the low basal levels of IL-6 promoter activity in L929sA fibrosarcoma cells and that RBP-Jkappa partially blocked access of NF-kappaB complexes to the IL-6 promoter. We propose that such a mechanism could be involved in the constitutive repression of the IL-6 gene under normal physiological conditions.
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Authors | S Plaisance, W Vanden Berghe, E Boone, W Fiers, G Haegeman |
Journal | Molecular and cellular biology
(Mol Cell Biol)
Vol. 17
Issue 7
Pg. 3733-43
(Jul 1997)
ISSN: 0270-7306 [Print] United States |
PMID | 9199307
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA-Binding Proteins
- Immunoglobulin J Recombination Signal Sequence-Binding Protein
- Interleukin-6
- NF-kappa B
- Nuclear Proteins
- Rbpj protein, mouse
- Repressor Proteins
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Topics |
- Animals
- Base Sequence
- Binding Sites
- Cell Line
- DNA-Binding Proteins
(physiology)
- Gene Expression Regulation
- Immunoglobulin J Recombination Signal Sequence-Binding Protein
- Interleukin-6
(genetics)
- Mice
- Mutagenesis, Site-Directed
- NF-kappa B
(physiology)
- Nuclear Proteins
(metabolism)
- Promoter Regions, Genetic
- Recombination, Genetic
- Repressor Proteins
(physiology)
- Transcription, Genetic
- Transcriptional Activation
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