HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Induction of humoral and cellular immune responses by vaccination with M. tuberculosis antigen 85 DNA.

Abstract
DNA vaccines have been demonstrated to be effective in inducing protective cell-mediated immune responses in animal models of infectious disease. In order to investigate this approach for potential use as a vaccine for tuberculosis, DNA constructs encoding Mycobacterium tuberculosis antigen 85A (Ag85A) were prepared. Expression of Ag85A in mammalian cells was demonstrated by transient transfection of cells in vitro. Intramuscular injection of Ag85A DNA vaccines resulted in the generation of anti-Ag85A antibodies and robust cell-mediated immune responses, as measured by lymphoproliferation of spleen cells in vitro upon specific antigen restimulation, leading to protection in animal challenge models. Therefore, the technique of DNA vaccination is effective in inducing relevant immune responses for protection against tuberculosis and may be used to identify the protective antigens of M. tuberculosis.
AuthorsD L Montgomery, K Huygen, A M Yawman, R R Deck, C M Dewitt, J Content, M A Liu, J B Ulmer
JournalCellular and molecular biology (Noisy-le-Grand, France) (Cell Mol Biol (Noisy-le-grand)) Vol. 43 Issue 3 Pg. 285-92 (May 1997) ISSN: 0145-5680 [Print] France
PMID9193782 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • DNA, Bacterial
  • Vaccines, DNA
Topics
  • Animals
  • Antibodies, Bacterial (biosynthesis)
  • Antigens, Bacterial (genetics)
  • Cell Line
  • DNA, Bacterial (administration & dosage, genetics, immunology)
  • Female
  • Immunity, Cellular
  • In Vitro Techniques
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred BALB C
  • Mycobacterium tuberculosis (immunology)
  • Spleen (immunology)
  • Transfection
  • Vaccination
  • Vaccines, DNA (administration & dosage, genetics, immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: